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Polypeptides with known repeating sequences of amino acids. Comparison of several methods used for the synthesis of poly‐γ‐ D ‐ and L ‐glutamylglycine and investigation of its serological reaction with antianthrax immune serum

Polypeptides with known repeating sequences of amino acids. Comparison of several methods used... To make a comparison of some of the various polymerization methods, poly‐γ‐D‐and L‐glutamylglycine were synthesized through the mixed anhydride, carbodiimide, and pentachlorophenyl active ester methods. This last method proved to be best, on the basis of weight‐average molecular weights (&Mmacr;w). A detailed study was also carried out to determine the optimum conditions for this method. The &Mmacr;w, values of the polymers were determined by the sedimentation equilibrium method; it was found that the pentachlorophenyl active ester gave &Mmacr;w values of up to 11, 500 but the mixed anhydride and carbodiimide methods gave &Mmacr;w, values of only about 1000. Tests with rabbit antianthrax immune serum showed that neither D‐ nor L‐isomer precipitated with the antiserum, but that, both were able to inhibit the precipitation of the antiserum by anthrax polypeptide. In addition, the D‐polymer gave more inhibition than the L, which would indicate a specificity of the antibody for D‐glutamic acid residues. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biopolymers Wiley

Polypeptides with known repeating sequences of amino acids. Comparison of several methods used for the synthesis of poly‐γ‐ D ‐ and L ‐glutamylglycine and investigation of its serological reaction with antianthrax immune serum

Biopolymers , Volume 6 (6) – Jun 1, 1968

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References (27)

Publisher
Wiley
Copyright
Copyright © 1968 John Wiley & Sons, Inc.
ISSN
0006-3525
eISSN
1097-0282
DOI
10.1002/bip.1968.360060606
pmid
5654615
Publisher site
See Article on Publisher Site

Abstract

To make a comparison of some of the various polymerization methods, poly‐γ‐D‐and L‐glutamylglycine were synthesized through the mixed anhydride, carbodiimide, and pentachlorophenyl active ester methods. This last method proved to be best, on the basis of weight‐average molecular weights (&Mmacr;w). A detailed study was also carried out to determine the optimum conditions for this method. The &Mmacr;w, values of the polymers were determined by the sedimentation equilibrium method; it was found that the pentachlorophenyl active ester gave &Mmacr;w values of up to 11, 500 but the mixed anhydride and carbodiimide methods gave &Mmacr;w, values of only about 1000. Tests with rabbit antianthrax immune serum showed that neither D‐ nor L‐isomer precipitated with the antiserum, but that, both were able to inhibit the precipitation of the antiserum by anthrax polypeptide. In addition, the D‐polymer gave more inhibition than the L, which would indicate a specificity of the antibody for D‐glutamic acid residues.

Journal

BiopolymersWiley

Published: Jun 1, 1968

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