Polymorphic microsatellites in the common cuttlefish Sepia officinalis (Cephalopoda)

Polymorphic microsatellites in the common cuttlefish Sepia officinalis (Cephalopoda) Cephalopods not only represent important components of many marine ecosystems and commercial fisheries ( Caddy & Rodhouse 1998 ), but also offer interesting possibilities for examining the evolution of complex mating behaviours in a marine invertebrate group. To date, the application of molecular genetic markers, so successful in addressing issues at population and individual level within other groups, has had limited success with many cephalopods, mainly due to low levels of marker variability (e.g. Brierley . 1993 ) or difficulties with marker development. Microsatellite DNA markers have exceptional utility in such circumstances (see Jarne & Lagoda 1996 ), and initial results ( Shaw & Boyle 1997 ; Shaw . 1999 ) indicate that they will be equally important for future studies of cephalopod biology. The current study reports an initial description of highly polymorphic microsatellite loci in the cuttlefish Sepia officinalis , suitable for use in population studies and the analysis of mating systems. Total DNA was obtained from arm tips of ethanol‐preserved cuttlefish using a standard high‐salt extraction. A 300–600 bp partial genomic library was constructed for S. officinalis using ligation of size‐selected Sau 3AI‐digested total DNA into pUC18/ Bam HI vector (Pharmacia), then transfection into DH5α‐competent http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Ecology Wiley

Polymorphic microsatellites in the common cuttlefish Sepia officinalis (Cephalopoda)

Molecular Ecology, Volume 9 (2) – Feb 1, 2000

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Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0962-1083
eISSN
1365-294X
D.O.I.
10.1046/j.1365-294x.2000.00804.x
Publisher site
See Article on Publisher Site

Abstract

Cephalopods not only represent important components of many marine ecosystems and commercial fisheries ( Caddy & Rodhouse 1998 ), but also offer interesting possibilities for examining the evolution of complex mating behaviours in a marine invertebrate group. To date, the application of molecular genetic markers, so successful in addressing issues at population and individual level within other groups, has had limited success with many cephalopods, mainly due to low levels of marker variability (e.g. Brierley . 1993 ) or difficulties with marker development. Microsatellite DNA markers have exceptional utility in such circumstances (see Jarne & Lagoda 1996 ), and initial results ( Shaw & Boyle 1997 ; Shaw . 1999 ) indicate that they will be equally important for future studies of cephalopod biology. The current study reports an initial description of highly polymorphic microsatellite loci in the cuttlefish Sepia officinalis , suitable for use in population studies and the analysis of mating systems. Total DNA was obtained from arm tips of ethanol‐preserved cuttlefish using a standard high‐salt extraction. A 300–600 bp partial genomic library was constructed for S. officinalis using ligation of size‐selected Sau 3AI‐digested total DNA into pUC18/ Bam HI vector (Pharmacia), then transfection into DH5α‐competent

Journal

Molecular EcologyWiley

Published: Feb 1, 2000

References

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