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P. Jarne, P. Lagoda (1996)
Microsatellites, from molecules to populations and back.Trends in ecology & evolution, 11 10
A. Brierley, P. Rodhouse, P. Rodhouse, J. Thorpe, M. Clarke (1993)
Genetic evidence of population heterogeneity and cryptic speciation in the ommastrephid squid Martialia hyadesi from the Patagonian Shelf and Antarctic Polar Frontal ZoneMarine Biology, 116
K. Rassmann, C. Schlötterer, D. Tautz (1991)
Isolation of simple‐sequence loci for use in polymerase chain reaction‐based DNA fingerprintingELECTROPHORESIS, 12
Caddy Caddy, Rodhouse Rodhouse (1998)
Cephalopod and groundfish landings: evidence for ecological change in global fisheries?Reviews in Fish Biology and Fisheries, 8
P. Shaw, P. Boyle (1997)
Multiple paternity within the brood of single females of Loligo forbesi (Cephalopoda : Loliginidae), demonstrated with microsatellite DNA markersMarine Ecology Progress Series, 160
Shaw Shaw, Pierce Pierce, Boyle Boyle (1999)
Subtle population structuring within a highly vagile marine invertebrate, the veined squid Loligo forbesi (Cephalopoda: Loliginidae) uncovered using microsatellite DNA markersMolecular Ecology, 8
P. Shaw, G. Pierce, P. Boyle (1999)
Subtle population structuring within a highly vagile marine invertebrate, the veined squid Loligo forbesi, demonstrated with microsatellite DNA markersMolecular Ecology, 8
Cephalopods not only represent important components of many marine ecosystems and commercial fisheries ( Caddy & Rodhouse 1998 ), but also offer interesting possibilities for examining the evolution of complex mating behaviours in a marine invertebrate group. To date, the application of molecular genetic markers, so successful in addressing issues at population and individual level within other groups, has had limited success with many cephalopods, mainly due to low levels of marker variability (e.g. Brierley . 1993 ) or difficulties with marker development. Microsatellite DNA markers have exceptional utility in such circumstances (see Jarne & Lagoda 1996 ), and initial results ( Shaw & Boyle 1997 ; Shaw . 1999 ) indicate that they will be equally important for future studies of cephalopod biology. The current study reports an initial description of highly polymorphic microsatellite loci in the cuttlefish Sepia officinalis , suitable for use in population studies and the analysis of mating systems. Total DNA was obtained from arm tips of ethanol‐preserved cuttlefish using a standard high‐salt extraction. A 300–600 bp partial genomic library was constructed for S. officinalis using ligation of size‐selected Sau 3AI‐digested total DNA into pUC18/ Bam HI vector (Pharmacia), then transfection into DH5α‐competent
Molecular Ecology – Wiley
Published: Feb 1, 2000
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