Plant Peroxisomes, Reactive Oxygen Metabolism and Nitric Oxide

Plant Peroxisomes, Reactive Oxygen Metabolism and Nitric Oxide In plant cells, as in most eukaryotic organisms, peroxisomes are probably the major sites of intracellular H2O2 production, as a result of their essentially oxidative type of metabolism. Like mitochondria and chloroplasts, peroxisomes also produce superoxide radicals (O2.−) and there are, at least, two sites of superoxide generation: one in the organelle matrix, the generating system being xanthine oxidase, and another site in the peroxisomal membranes dependent on NAD(P)H. In peroxisomal membranes, three integral polypeptides (PMPs) with molecular masses of 18, 29, and 32 kDa have been shown to generate O2.− radicals. Besides catalase, several antioxidative systems have been demonstrated in plant peroxisomes, including different superoxide dismutases, the four enzymes of the ascorbate‐glutathione cycle plus ascorbate and glutathione, and three NADP‐dependent dehydrogenases. A CuZn‐SOD and two Mn‐SODs have been purified and characterized from different types of plant peroxisomes. The presence of the enzyme nitric oxide synthase (NOS) and its reaction product, nitric oxide (NO.), has been recently demonstrated in plant peroxisomes. Different experimental evidence has suggested that peroxisomes have a ROS‐mediated cellular function in leaf senescence and in stress situations induced by xenobiotics and heavy metals. Peroxisomes could also have a role in plant cells as a source of signal molecules like NO., O2.− radicals, H2O2, and possibly S‐nitrosoglutathione (GSNO). It seems reasonable to think that a signal molecule‐producing function similar to that postulated for plant peroxisomes could also be performed by human, animal and yeast peroxisomes, where research on oxy radicals, antioxidants and nitric oxide is less advanced than in plant peroxisomes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png IUBMB Life Wiley

Plant Peroxisomes, Reactive Oxygen Metabolism and Nitric Oxide

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2003 International Union of Biochemistry and Molecular Biology
ISSN
1521-6543
eISSN
1521-6551
DOI
10.1002/tbmb.718540875
Publisher site
See Article on Publisher Site

Abstract

In plant cells, as in most eukaryotic organisms, peroxisomes are probably the major sites of intracellular H2O2 production, as a result of their essentially oxidative type of metabolism. Like mitochondria and chloroplasts, peroxisomes also produce superoxide radicals (O2.−) and there are, at least, two sites of superoxide generation: one in the organelle matrix, the generating system being xanthine oxidase, and another site in the peroxisomal membranes dependent on NAD(P)H. In peroxisomal membranes, three integral polypeptides (PMPs) with molecular masses of 18, 29, and 32 kDa have been shown to generate O2.− radicals. Besides catalase, several antioxidative systems have been demonstrated in plant peroxisomes, including different superoxide dismutases, the four enzymes of the ascorbate‐glutathione cycle plus ascorbate and glutathione, and three NADP‐dependent dehydrogenases. A CuZn‐SOD and two Mn‐SODs have been purified and characterized from different types of plant peroxisomes. The presence of the enzyme nitric oxide synthase (NOS) and its reaction product, nitric oxide (NO.), has been recently demonstrated in plant peroxisomes. Different experimental evidence has suggested that peroxisomes have a ROS‐mediated cellular function in leaf senescence and in stress situations induced by xenobiotics and heavy metals. Peroxisomes could also have a role in plant cells as a source of signal molecules like NO., O2.− radicals, H2O2, and possibly S‐nitrosoglutathione (GSNO). It seems reasonable to think that a signal molecule‐producing function similar to that postulated for plant peroxisomes could also be performed by human, animal and yeast peroxisomes, where research on oxy radicals, antioxidants and nitric oxide is less advanced than in plant peroxisomes.

Journal

IUBMB LifeWiley

Published: Feb 1, 2003

Keywords: Antioxidants; nitric oxide; oxidative stress; peroxisomes; reactive oxygen species; ROS; signalling

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