PHOTODYNAMIC ACTION OF MEROCYANINE 540 IN ARTIFICIAL BILAYERS AND NATURAL MEMBRANES: ACTION SPECTRA AND QUANTUM YIELDS

PHOTODYNAMIC ACTION OF MEROCYANINE 540 IN ARTIFICIAL BILAYERS AND NATURAL MEMBRANES: ACTION... Abstract— The action spectra and quantum yields for singlet oxygen (1O2) generation by merocyanine 540 (MC540) in liposomes and isolated erythrocyte membranes were obtained using electron spin resonance techniques. Oxygen consumption was measured by spin label oximetry in the presence of histidine for fully‐saturated dimyristoylphosphatidylcholine vesicles, mono‐unsaturated 1‐palmitoyl‐2‐oleoylphosphatidylcholine vesicles and erythrocyte membranes. The quantum yield for the photogeneration of 1O2 by membrane‐bound MC540 in aqueous buffer was determined to be 0.065 ± 0.005, which is approx. 1/10 of the value determined for Rose Bengal under similar conditions. Using unilamellar liposomes and isolated erythrocyte membranes containing MC540 at different monomer/dimer ratios, we have observed that the action spectra of 1O2 generation closely overlap the absorption spectra of the monomeric dye in these systems. It is likely that factors which affect the monomer‐dimer equilibrium of MC540 will influence the production of 1O2. These findings have important implications for the phototherapeutic efficacy of MC540. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Photochemistry & Photobiology Wiley

PHOTODYNAMIC ACTION OF MEROCYANINE 540 IN ARTIFICIAL BILAYERS AND NATURAL MEMBRANES: ACTION SPECTRA AND QUANTUM YIELDS

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Publisher
Wiley
Copyright
Copyright © 1991 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0031-8655
eISSN
1751-1097
DOI
10.1111/j.1751-1097.1991.tb03661.x
Publisher site
See Article on Publisher Site

Abstract

Abstract— The action spectra and quantum yields for singlet oxygen (1O2) generation by merocyanine 540 (MC540) in liposomes and isolated erythrocyte membranes were obtained using electron spin resonance techniques. Oxygen consumption was measured by spin label oximetry in the presence of histidine for fully‐saturated dimyristoylphosphatidylcholine vesicles, mono‐unsaturated 1‐palmitoyl‐2‐oleoylphosphatidylcholine vesicles and erythrocyte membranes. The quantum yield for the photogeneration of 1O2 by membrane‐bound MC540 in aqueous buffer was determined to be 0.065 ± 0.005, which is approx. 1/10 of the value determined for Rose Bengal under similar conditions. Using unilamellar liposomes and isolated erythrocyte membranes containing MC540 at different monomer/dimer ratios, we have observed that the action spectra of 1O2 generation closely overlap the absorption spectra of the monomeric dye in these systems. It is likely that factors which affect the monomer‐dimer equilibrium of MC540 will influence the production of 1O2. These findings have important implications for the phototherapeutic efficacy of MC540.

Journal

Photochemistry & PhotobiologyWiley

Published: Apr 1, 1991

References

  • The use of spin label oximetry in the study of photodynamic inactivation of Chinese hamster ovary cells
    Ankel, Ankel; Felix, Felix; Kalyanaraman, Kalyanaraman
  • The photophysics of merocyanine 540. A comparative study in ethanol and in liposomes
    Aramendia, Aramendia; Kreig, Kreig; Nitsch, Nitsch; Bittersmann, Bittersmann; Braslavsky, Braslavsky
  • Quantum yield of singlet oxygen production by xanthene derivatives
    Gandin, Gandin; Lion, Lion; Vorst, Vorst
  • Determination of singlet oxygen rate constants in aqueous solutions
    Kraljic, Kraljic; Sharpatyi, Sharpatyi
  • Photoinduced oxygen consumption in Melanin Systems. Action Spectra and Quantum yields for eumelanins and synthetic melanin
    Sarna, Sarna; Sealy, Sealy

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