Persistent parvovirus B19 infections with different clinical outcomes in renal transplant recipients: diagnostic relevance of polymerase chain reaction (PCR) and of quantification of B19 DNA in sera

Persistent parvovirus B19 infections with different clinical outcomes in renal transplant... Objective: To study parvovirus B19 infection in immunocompromised subjects such as renal transplantation recipients. Methods: Two cases of B19 infection in renal transplant recipients have been included in the study. The outcome of the infection has been studied by both serologic and virologic methods. A monitoring of the DNAemia was done by a nested PCR in endpoint titration assays. Results: In one patient with severe anemia an acute B19 infection was diagnosed by PCR 26 days after the transplant; a high level of DNAemia persisted until an intravenous immunoglobulin treatment. Then a sharp decrease of the DNAemia was shown, without full clearance of B19 virus. In a lymphocyte suspension from the organ donor, B19 DNA was detected. In the other patient, who recovered spontaneously from anemia, a persistent B19 infection was demonstrated at day 106 after transplantation and was still demonstrable after 470 days. Conclusions: A high level of B19 DNAemia was associated with symptomatic infection, with severe anemia, whereas low‐level DNAemia was long‐lasting in asymptomatic subjects with impaired immunologic responses. The endpoint titration assay by nested PCR was very useful for the monitoring of B19 infection, particularly following the therapeutic intravenous immunoglobulin administration. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Microbiology and Infection Wiley

Persistent parvovirus B19 infections with different clinical outcomes in renal transplant recipients: diagnostic relevance of polymerase chain reaction (PCR) and of quantification of B19 DNA in sera

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Publisher
Wiley
Copyright
1996 European Society of Clinical Microbiology and Infectious Diseases
ISSN
1198-743X
eISSN
1469-0691
D.O.I.
10.1111/j.1469-0691.1996.tb00214.x
Publisher site
See Article on Publisher Site

Abstract

Objective: To study parvovirus B19 infection in immunocompromised subjects such as renal transplantation recipients. Methods: Two cases of B19 infection in renal transplant recipients have been included in the study. The outcome of the infection has been studied by both serologic and virologic methods. A monitoring of the DNAemia was done by a nested PCR in endpoint titration assays. Results: In one patient with severe anemia an acute B19 infection was diagnosed by PCR 26 days after the transplant; a high level of DNAemia persisted until an intravenous immunoglobulin treatment. Then a sharp decrease of the DNAemia was shown, without full clearance of B19 virus. In a lymphocyte suspension from the organ donor, B19 DNA was detected. In the other patient, who recovered spontaneously from anemia, a persistent B19 infection was demonstrated at day 106 after transplantation and was still demonstrable after 470 days. Conclusions: A high level of B19 DNAemia was associated with symptomatic infection, with severe anemia, whereas low‐level DNAemia was long‐lasting in asymptomatic subjects with impaired immunologic responses. The endpoint titration assay by nested PCR was very useful for the monitoring of B19 infection, particularly following the therapeutic intravenous immunoglobulin administration.

Journal

Clinical Microbiology and InfectionWiley

Published: Oct 1, 1996

References

  • Nested polymerase chain reaction assay for the detection of B19 parvovirus DNA in human immunodeficiency patients
    Musiani, Musiani; Azzi, Azzi; Zerbini, Zerbini

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