Persistent parvovirus B19 infection with a low viral load has been reported in immunocompromised and in immunocompetent individuals (reviewed in Parsyan and Candotti ). Large cross‐sectional studies using highly sensitive DNA amplification methods have also demonstrated persistent B19 infection. Recently, Lefere and colleagues conducted a longitudinal study of nonimmunodeficient patients who were multitransfused with red blood cells, demonstrating that asymptomatic chronic B19 infections may persist for a long period. To characterize the natural course of persistent B19 infections, we conducted the following longitudinal study using an in‐house TaqMan polymerase chain reaction method for B19 DNA and enzyme immunoassays to detect B19 immunoglobulin M (IgM) and immunoglobulin G (IgG; Denka Seiken, Tokyo, Japan). This study was approved by the ethical Committee of the Japanese Red Cross Osaka Blood Center. In Japan, all donated blood is tested for B19 infection with an in‐house receptor‐mediated hemagglutination method that detects B19 antigen as a marker of a high viremic stage of infection (cutoff, approx. 2.5 × 10 10 IU/mL B19 DNA; data not shown). Using this method, we identified 102 cases of B19 infection among 979,052 blood donors in Osaka between 1997 and 1999. We were able to test the plasma samples
Transfusion – Wiley
Published: May 1, 2008
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