Peptide selection for the quantification of P‐III‐NP in human serum by mass spectrometry

Peptide selection for the quantification of P‐III‐NP in human serum by mass spectrometry INTRODUCTIONType III collagen is the second most abundant collagen (varying in abundance with age) in humans; it is typically found with type I collagen and is present in tissues with elastic properties such as skin, blood vessels and various internal organs. Type III collagen is formed from a precursor pro‐collagen unit in the cell, which includes amino‐ and carboxyl‐terminal extension propeptides (denoted P‐III‐NP and P‐III‐CP, respectively).P‐III‐NP is a clinical biomarker used to monitor chronic active hepatitis, liver fibrosis and cirrhosis. Elevated levels of P‐III‐NP have been associated with hormonally induced growth, making P‐III‐NP one of two biomarkers used to detect rhGH (recombinant human growth hormone) doping in sport; the other being insulin‐like growth factor I (IGF‐I). These compounds have little diurnal variation and are largely unaffected by exercise or sex, but respond to hGH administration. Also, body mass index and race contribute little to the variability of these markers. For humans, blood P‐III‐NP concentrations are inversely proportional to age, with maximum concentrations being observed during pubertal years. Sex however affects P‐III‐NP blood concentrations, men (average conc. 5.4 ± 2.3 ng/mL) having a slightly larger natural concentration than women (average conc. 5.1 ± 1.5 ng/mL).P‐III‐NP consists of three identical 130 amino acid http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Rapid Communications in Mass Spectrometry Wiley

Peptide selection for the quantification of P‐III‐NP in human serum by mass spectrometry

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons, Ltd.
ISSN
0951-4198
eISSN
1097-0231
D.O.I.
10.1002/rcm.8066
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONType III collagen is the second most abundant collagen (varying in abundance with age) in humans; it is typically found with type I collagen and is present in tissues with elastic properties such as skin, blood vessels and various internal organs. Type III collagen is formed from a precursor pro‐collagen unit in the cell, which includes amino‐ and carboxyl‐terminal extension propeptides (denoted P‐III‐NP and P‐III‐CP, respectively).P‐III‐NP is a clinical biomarker used to monitor chronic active hepatitis, liver fibrosis and cirrhosis. Elevated levels of P‐III‐NP have been associated with hormonally induced growth, making P‐III‐NP one of two biomarkers used to detect rhGH (recombinant human growth hormone) doping in sport; the other being insulin‐like growth factor I (IGF‐I). These compounds have little diurnal variation and are largely unaffected by exercise or sex, but respond to hGH administration. Also, body mass index and race contribute little to the variability of these markers. For humans, blood P‐III‐NP concentrations are inversely proportional to age, with maximum concentrations being observed during pubertal years. Sex however affects P‐III‐NP blood concentrations, men (average conc. 5.4 ± 2.3 ng/mL) having a slightly larger natural concentration than women (average conc. 5.1 ± 1.5 ng/mL).P‐III‐NP consists of three identical 130 amino acid

Journal

Rapid Communications in Mass SpectrometryWiley

Published: Jan 15, 2018

References

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