Peptidase activity in various species of dairy thermophilic lactobacilli

Peptidase activity in various species of dairy thermophilic lactobacilli Aims: The aim of the present work was to evaluate the enzymatic potential manifested by aminopeptidase activity of different thermophilic Lactobacillus biotypes and to measure the influence of cell growth phase on enzyme expression. Methods and Results: The activities were evaluated by the hydrolysis of β‐naphthylamide substrates for both whole and mechanically disrupted cells of L. helveticus, L. delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis strains, collected from both the exponential and the stationary growth phase. In general, activities were higher for cells in the exponential rather than in the stationary phase and the disrupted cells showed higher activities than the whole cells. The highest activity expressed by all strains corresponded to X‐prolyl‐dipeptidyl aminopeptidase while a moderate activity was observed towards Arg‐βNa, Lys‐βNa and Leu‐βNa. The lowest activity was observed for Pro‐βNa. Conclusions: It may be inferred that the cell structure and the cell physiology are crucial to define the level of efficiency of expression for aminopeptidase activity. The two species may be characterized by a different enzymatic system that hydrolyses N‐terminal leucine. Significance and Impact of the Study: The differences of peptidase activities in L. helveticus and L. delbrueckii species acquires an importance to comprehend their role in the biochemical events occurring in cheese ripening. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Microbiology Wiley

Peptidase activity in various species of dairy thermophilic lactobacilli

Loading next page...
 
/lp/wiley/peptidase-activity-in-various-species-of-dairy-thermophilic-WQA3sukcy0
Publisher
Wiley
Copyright
Copyright © 2004 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1364-5072
eISSN
1365-2672
D.O.I.
10.1046/j.1365-2672.2003.02142.x
Publisher site
See Article on Publisher Site

Abstract

Aims: The aim of the present work was to evaluate the enzymatic potential manifested by aminopeptidase activity of different thermophilic Lactobacillus biotypes and to measure the influence of cell growth phase on enzyme expression. Methods and Results: The activities were evaluated by the hydrolysis of β‐naphthylamide substrates for both whole and mechanically disrupted cells of L. helveticus, L. delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis strains, collected from both the exponential and the stationary growth phase. In general, activities were higher for cells in the exponential rather than in the stationary phase and the disrupted cells showed higher activities than the whole cells. The highest activity expressed by all strains corresponded to X‐prolyl‐dipeptidyl aminopeptidase while a moderate activity was observed towards Arg‐βNa, Lys‐βNa and Leu‐βNa. The lowest activity was observed for Pro‐βNa. Conclusions: It may be inferred that the cell structure and the cell physiology are crucial to define the level of efficiency of expression for aminopeptidase activity. The two species may be characterized by a different enzymatic system that hydrolyses N‐terminal leucine. Significance and Impact of the Study: The differences of peptidase activities in L. helveticus and L. delbrueckii species acquires an importance to comprehend their role in the biochemical events occurring in cheese ripening.

Journal

Journal of Applied MicrobiologyWiley

Published: Feb 1, 2004

References

  • The influence of phage‐assisted lysis of Lactococcus lactis subsp. lactis ML8 on Cheddar cheese ripening
    Crow, Crow; Martley, Martley; Coolbear, Coolbear; Roundhill, Roundhill
  • Presence of peptidase activities in different varieties of cheese
    Gatti, Gatti; Fornasari, Fornasari; Mucchetti, Mucchetti; Addeo, Addeo; Neviani, Neviani

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off