PCR‐RFLP of the mitochondrial cytochrome oxidase gene: a simple method for discrimination between Atlantic salmon ( Salmo salar ) and rainbow trout ( Oncorhynchus mykiss )

PCR‐RFLP of the mitochondrial cytochrome oxidase gene: a simple method for discrimination... A DNA‐based method (PCR‐RFLP) has been developed for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). The polymerase chain reaction (PCR) was used for amplification of a 464 bp fragment of the mitochondrial cytochrome oxidase subunit II (COII) gene. Digestion of the products with endonucleases Nci I and Sau 3AI, followed by agarose gel electrophoresis of the digested products, yielded specific restriction profiles that enabled direct visual identification of the species analysed. This PCR‐RFLP methodology allowed clear discrimination of Atlantic salmon and rainbow trout samples both in raw and smoked products. © 1999 Society of Chemical Industry http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of the Science of Food and Agriculture Wiley

PCR‐RFLP of the mitochondrial cytochrome oxidase gene: a simple method for discrimination between Atlantic salmon ( Salmo salar ) and rainbow trout ( Oncorhynchus mykiss )

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Publisher
Wiley
Copyright
Copyright © 1999 Society of Chemical Industry
ISSN
0022-5142
eISSN
1097-0010
D.O.I.
10.1002/(SICI)1097-0010(199909)79:12<1654::AID-JSFA414>3.0.CO;2-S
Publisher site
See Article on Publisher Site

Abstract

A DNA‐based method (PCR‐RFLP) has been developed for discrimination between Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). The polymerase chain reaction (PCR) was used for amplification of a 464 bp fragment of the mitochondrial cytochrome oxidase subunit II (COII) gene. Digestion of the products with endonucleases Nci I and Sau 3AI, followed by agarose gel electrophoresis of the digested products, yielded specific restriction profiles that enabled direct visual identification of the species analysed. This PCR‐RFLP methodology allowed clear discrimination of Atlantic salmon and rainbow trout samples both in raw and smoked products. © 1999 Society of Chemical Industry

Journal

Journal of the Science of Food and AgricultureWiley

Published: Sep 1, 1999

References

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