PCR‐RFLP Analysis of Mitochondrial DNA for Identification of Snail Meat Species

PCR‐RFLP Analysis of Mitochondrial DNA for Identification of Snail Meat Species ABSTRACT Several species of land snails, including Helix pomatia and Helix lucorum are consumed as food products. The main source of commercial competition is an imported African snail, Achatina fulica. The only way to distinguish between these species has been morphologically. We hypothesized a reliable method for identifying canned snails could be based on using PCR‐RFLP analysis of mito‐chondrial DNA. The molecular weights of the amplified fragments were perfectly identical, regardless of low extraction (fresh snails or cooked and canned samples). The whole amplified products (16S rRNA and 12S rRNA) made it possible to check any fraudulent label and identify the three species using four restriction enzymes (RFLP). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Food Science Wiley

PCR‐RFLP Analysis of Mitochondrial DNA for Identification of Snail Meat Species

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Abstract

ABSTRACT Several species of land snails, including Helix pomatia and Helix lucorum are consumed as food products. The main source of commercial competition is an imported African snail, Achatina fulica. The only way to distinguish between these species has been morphologically. We hypothesized a reliable method for identifying canned snails could be based on using PCR‐RFLP analysis of mito‐chondrial DNA. The molecular weights of the amplified fragments were perfectly identical, regardless of low extraction (fresh snails or cooked and canned samples). The whole amplified products (16S rRNA and 12S rRNA) made it possible to check any fraudulent label and identify the three species using four restriction enzymes (RFLP).

Journal

Journal of Food ScienceWiley

Published: Jan 1, 1996

References

  • Differentiation between Helix and Achatina snail meat by gel electrophoresis
    Bracchi, Bracchi

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