Department of Virology, Faculty of Medicine (K.U.), and School of Health Sciences (T.N.),Kyushu Uniuersity, Fukuoka, Japan Kurtzman et al., 1988; Mortimer et al., 1985; Nascimento et al., 1991; Nunoue et al., 1985, 1987; Smith et al., 1988; Weiland et al., 19871. The B19 viral particle contains a 5.6 kb,linear, single-stranded DNA genome of either polarity, with terminal palindromic sequences of 383 nucleotides (nt) [Clewley, 1984; Deiss et al., 1990; Shade et al., 1986; Summers et al., 19831. The nucleotide sequences of the almost-full-length genome of two B19 strains have been determined [Blundell et al., 1987; Shade et al., 19861. B19 is difficult to grow in the laboratory; it does not propagate in conventional cell lines [Shimomura et al., 19921. Productive B19 infection occurs in human erythroid progenitor cells, and B19 has been propagated in suspension cultures of human erythroid bone marrow cells [Ozawa et al., 19861, in primary human fetal liver culture [Yaegashi et al., 19891, in hematopoietic progenitor cells generated in vitro from normal human peripheral blood [Schwarz et al., 19921, and in human umbilical cord blood erythroid progenitor cells [Sosa et al., 19921. Because these cells cannot be used conventionally, amplification by the polymerase chain reaction
Journal of Medical Virology – Wiley
Published: Apr 1, 1993
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