Department of Virology, Faculty of Medicine (K.U.), and School of Health Sciences (T.N.),Kyushu Uniuersity, Fukuoka, Japan Kurtzman et al., 1988; Mortimer et al., 1985; Nascimento et al., 1991; Nunoue et al., 1985, 1987; Smith et al., 1988; Weiland et al., 19871. The B19 viral particle contains a 5.6 kb,linear, single-stranded DNA genome of either polarity, with terminal palindromic sequences of 383 nucleotides (nt) [Clewley, 1984; Deiss et al., 1990; Shade et al., 1986; Summers et al., 19831. The nucleotide sequences of the almost-full-length genome of two B19 strains have been determined [Blundell et al., 1987; Shade et al., 19861. B19 is difficult to grow in the laboratory; it does not propagate in conventional cell lines [Shimomura et al., 19921. Productive B19 infection occurs in human erythroid progenitor cells, and B19 has been propagated in suspension cultures of human erythroid bone marrow cells [Ozawa et al., 19861, in primary human fetal liver culture [Yaegashi et al., 19891, in hematopoietic progenitor cells generated in vitro from normal human peripheral blood [Schwarz et al., 19921, and in human umbilical cord blood erythroid progenitor cells [Sosa et al., 19921. Because these cells cannot be used conventionally, amplification by the polymerase chain reaction
Journal of Medical Virology – Wiley
Published: Apr 1, 1993
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera