Over‐expression of secreted proteins from mammalian cell lines

Over‐expression of secreted proteins from mammalian cell lines Secreted mammalian proteins require the development of robust protein over‐expression systems for crystallographic and biophysical studies of protein function. Due to complex disulfide bonds and distinct glycosylation patterns preventing folding and expression in prokaryotic expression hosts, many secreted proteins necessitate production in more complex eukaryotic expression systems. Here, we elaborate on the methods used to obtain high yields of purified secreted proteins from transiently or stably transfected mammalian cell lines. Among the issues discussed are the selection of appropriate expression vectors, choice of signal sequences for protein secretion, availability of fusion tags for enhancing protein stability and purification, choice of cell line, and the large‐scale growth of cells in a variety of formats. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Protein Science Wiley

Over‐expression of secreted proteins from mammalian cell lines

Protein Science, Volume 23 (5) – Jan 1, 2014

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Publisher
Wiley
Copyright
© 2014 The Protein Society
ISSN
0961-8368
eISSN
1469-896X
D.O.I.
10.1002/pro.2439
Publisher site
See Article on Publisher Site

Abstract

Secreted mammalian proteins require the development of robust protein over‐expression systems for crystallographic and biophysical studies of protein function. Due to complex disulfide bonds and distinct glycosylation patterns preventing folding and expression in prokaryotic expression hosts, many secreted proteins necessitate production in more complex eukaryotic expression systems. Here, we elaborate on the methods used to obtain high yields of purified secreted proteins from transiently or stably transfected mammalian cell lines. Among the issues discussed are the selection of appropriate expression vectors, choice of signal sequences for protein secretion, availability of fusion tags for enhancing protein stability and purification, choice of cell line, and the large‐scale growth of cells in a variety of formats.

Journal

Protein ScienceWiley

Published: Jan 1, 2014

Keywords: ; ; ;

References

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