Osmostress‐induced changes in yeast gene expression

Osmostress‐induced changes in yeast gene expression Summary When Saccharomyces cerevisiae cells are exposed to high concentrations of NaCI, they show reduced viability, methionine uptake and protein biosynthesis. Cells can acquire tolerance against a severe salt shock (up to 1.4 M NaCI) by a previous treatment with 0.7 M NaCI, but not by a previous heat shock. Two‐dimensional analysis of (3H)‐leucine‐labelled proteins from salt‐shocked cells (0.7 M NaCt) revealed the elevated rate of synthesis of nine proteins, among which were the heat‐shock proteins hsp12 and hsp26. Northern analysis using gene‐specific probes confirmed the identity of the latter proteins and, in addition, demonstrated the induction of glycerol‐3‐phos‐phate dehydrogenase gene expression. The synthesis of the same set of proteins is induced or enhanced upon exposure of cells to 0.8 M sucrose, although not as dramatically as in an iso‐osmolar NaCI concentration (0.7 M). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Microbiology Wiley

Osmostress‐induced changes in yeast gene expression

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Publisher
Wiley
Copyright
Copyright © 1992 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0950-382X
eISSN
1365-2958
D.O.I.
10.1111/j.1365-2958.1992.tb01392.x
Publisher site
See Article on Publisher Site

Abstract

Summary When Saccharomyces cerevisiae cells are exposed to high concentrations of NaCI, they show reduced viability, methionine uptake and protein biosynthesis. Cells can acquire tolerance against a severe salt shock (up to 1.4 M NaCI) by a previous treatment with 0.7 M NaCI, but not by a previous heat shock. Two‐dimensional analysis of (3H)‐leucine‐labelled proteins from salt‐shocked cells (0.7 M NaCt) revealed the elevated rate of synthesis of nine proteins, among which were the heat‐shock proteins hsp12 and hsp26. Northern analysis using gene‐specific probes confirmed the identity of the latter proteins and, in addition, demonstrated the induction of glycerol‐3‐phos‐phate dehydrogenase gene expression. The synthesis of the same set of proteins is induced or enhanced upon exposure of cells to 0.8 M sucrose, although not as dramatically as in an iso‐osmolar NaCI concentration (0.7 M).

Journal

Molecular MicrobiologyWiley

Published: Aug 1, 1992

References

  • Induction of stress proteins by sodium chloride treatment in Bacillus subtilis
    Hecker, Hecker; Helm, Helm; Volker, Volker; Wolfel, Wolfel
  • Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the porin genes
    Mizuno, Mizuno; Mizushima, Mizushima

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