Oral immunization with whole yeast producing viral capsid antigen provokes a stronger humoral immune response than purified viral capsid antigen

Oral immunization with whole yeast producing viral capsid antigen provokes a stronger humoral... Weak antibody responses to protein antigens after oral immunization remain a serious problem. Yeasts have a rigid cell wall and are inherently resistant to harsh conditions, suggesting that recombinant antigens made in yeast could have a greater chance of making contact with the immune cells of the gastrointestinal (GI) tract in intact form. We compared antibody responses to oral immunization with purified recombinant antigen, used in the conventional manner, and responses to whole recombinant yeast producing the antigen intracellularly. Recombinant capsid protein (CP) of red‐spotted grouper necrosis virus (RGNNV) was used as model antigen and Saccharomyces cerevisiae as host. The purified CP was obtained from the S. cerevisiae producing the RGNNV CP. Whole recombinant yeast producing RGNNV CP provoked 9–27 times higher anti‐RGNNV CP IgG titres than purified RGNNV CP. Moreover, sera from mice immunized with the recombinant yeast had neutralizing activity against RGNNV, while those from mice immunized with purified CP did not. These results show that whole recombinant yeast is a promising platform for antigen delivery by oral immunization. Significance and Impact of the Study Provoking sufficient antibody responses by oral immunization has been an enormous challenge because of the harsh conditions of the gastrointestinal (GI) tract. Immunization strategies using purified antigen to make oral vaccines are incapable of commercialization because excessive amount of antigen is required to provoke antibody responses. Therefore, resolving the problems concerning the cost and effectiveness of oral vaccines is a high priority. Our results suggest that recombinant yeast has great potential for inducing antigen‐specific immune responses by oral immunization. We believe that oral immunization using recombinant yeast can be a breakthrough technology. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Letters in Applied Microbiology Wiley

Oral immunization with whole yeast producing viral capsid antigen provokes a stronger humoral immune response than purified viral capsid antigen

Loading next page...
 
/lp/wiley/oral-immunization-with-whole-yeast-producing-viral-capsid-antigen-7kZISo1893
Publisher
Wiley
Copyright
Copyright © 2014 The Society for Applied Microbiology
ISSN
0266-8254
eISSN
1472-765X
D.O.I.
10.1111/lam.12188
Publisher site
See Article on Publisher Site

Abstract

Weak antibody responses to protein antigens after oral immunization remain a serious problem. Yeasts have a rigid cell wall and are inherently resistant to harsh conditions, suggesting that recombinant antigens made in yeast could have a greater chance of making contact with the immune cells of the gastrointestinal (GI) tract in intact form. We compared antibody responses to oral immunization with purified recombinant antigen, used in the conventional manner, and responses to whole recombinant yeast producing the antigen intracellularly. Recombinant capsid protein (CP) of red‐spotted grouper necrosis virus (RGNNV) was used as model antigen and Saccharomyces cerevisiae as host. The purified CP was obtained from the S. cerevisiae producing the RGNNV CP. Whole recombinant yeast producing RGNNV CP provoked 9–27 times higher anti‐RGNNV CP IgG titres than purified RGNNV CP. Moreover, sera from mice immunized with the recombinant yeast had neutralizing activity against RGNNV, while those from mice immunized with purified CP did not. These results show that whole recombinant yeast is a promising platform for antigen delivery by oral immunization. Significance and Impact of the Study Provoking sufficient antibody responses by oral immunization has been an enormous challenge because of the harsh conditions of the gastrointestinal (GI) tract. Immunization strategies using purified antigen to make oral vaccines are incapable of commercialization because excessive amount of antigen is required to provoke antibody responses. Therefore, resolving the problems concerning the cost and effectiveness of oral vaccines is a high priority. Our results suggest that recombinant yeast has great potential for inducing antigen‐specific immune responses by oral immunization. We believe that oral immunization using recombinant yeast can be a breakthrough technology.

Journal

Letters in Applied MicrobiologyWiley

Published: Mar 1, 2014

References

  • Chromatographically‐purified capsid proteins of red‐spotted grouper nervous necrosis virus expressed in Saccharomyces cerevisiae form virus‐like particles
    Choi, Choi; Kim, Kim; Lee, Lee; Kang, Kang; Kim, Kim
  • Disassembly and reassembly of yeast‐derived recombinant human papillomavirus virus‐like particles (HPV VLPs)
    Mach, Mach; Volkin, Volkin; Troutman, Troutman; Wang, Wang; Luo, Luo; Jansen, Jansen; Shi, Shi
  • Nodaviruses as pathogens in larval and juvenile marine finfish
    Munday, Munday; Nakai, Nakai
  • Phylogenetic and antigenic characterization of new fish nodavirus isolates from Europe and Asia
    Skliris, Skliris; Krondiris, Krondiris; Sideris, Sideris; Shinn, Shinn; Starkey, Starkey; Richards, Richards
  • Pathogen recognition and development of particulate vaccines: does size matter?
    Xiang, Xiang; Scholzen, Scholzen; Minigo, Minigo; David, David; Apostolopoulos, Apostolopoulos; Mottram, Mottram; Plebanski, Plebanski

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off