Intracellular recordings were made from locus coeruleus neurones in slices cut from rat pons and superfused in vitro. Membrane currents were recorded with a single‐electrode switch‐clamp amplifier. Opioids, enkephalin analogues or morphine, caused a concentration‐dependent potassium current, which had a maximum value of about 300 pA at ‐60 mV. The opioid‐sensitive potassium conductance was independent of membrane potential between ‐60 and ‐130 mV, but became less as the membrane potential was changed from ‐60 to ‐30 mV. The opioid outward current was reduced by quinine (100 microM‐1 mM) and barium (30 microM‐2 mM), but not by 4‐aminopyridine (100 microM‐1 mM) or tetraethylammonium (10 mM). A potassium current with similar properties flowed for several seconds after a burst of action potentials; this appeared to result from calcium entering the neurone during the action potentials. The alpha 2‐adrenoceptor agonists noradrenaline and clonidine caused a concentration‐dependent potassium conductance increase which had the same maximum value as that caused by opioids in the same neurones. Experiments in which an opioid and an alpha 2‐adrenoceptor agonist were superfused together indicated that the same potassium conductance is increased by both agonists.
The Journal of Physiology – Wiley
Published: Jul 1, 1985
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera