Purpose and Appropriate Sample TypesThis 21‐color flow cytometry‐based OMIP enables simultaneous quantification of monocytes, basophils, granulocytes, dendritic cells, natural killer cells, B cells, and all well‐defined T and T helper cell subsets in the human peripheral blood (Table ). This panel captures the major phenotypes described in the NIH Human Immunology Project with additional markers for deep T cell analysis . We specifically designed this panel for analysis of peripheral blood from patients involved in our clinical trials of novel agents for the treatment of graft versus host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (alloHSCT). We have optimized this panel for the analysis of 1 × 106 fresh or previously frozen peripheral blood mononuclear cells (PBMCs).Summary table for OMIP‐042PurposeMyeloid and lymphoid comprehensive immunophenotypingCell typesHuman PBMCsCross‐referenceOMIP‐030, OMIP‐015, OMIP‐024BackgroundWe initially designed this panel for the analysis of the PBMCs from patients who have undergone alloHSCT, particularly those enrolled in drug studies for the prevention and treatment of GVHD. Prior studies in humans and animal models have implicated many immune cell types in the initiation and progression of GVHD, and the data have, at times, conflicted, depending on species, model, and individual laboratories. In particular, prior studies have identified imbalances
Cytometry – Wiley
Published: Jan 1, 2018
Keywords: ; ; ; ; ; ;
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