Nuclear import of the capsid protein of tomato yellow leaf curl virus (TYLCV) in plant and insect cells

Nuclear import of the capsid protein of tomato yellow leaf curl virus (TYLCV) in plant and insect... Summary The tomato yellow leaf curl virus (TYLCV) found in Israel is a whitefly‐transmitted monopartite geminivirus. Although geminiviruses have been found in the nuclei of phloem‐associated cells, the mechanism of viral invasion is poorly understood. The possible role of the TYLCV capsid protein (CP), the only known component of the viral coat, in virus transport into the host cell nucleus was investigated by monitoring its specific nuclear accumulation in plant and insect cells. CP was fused to the β‐glucuronidase (GUS) reporter enzyme to assay nuclear import in petunia protoplasts, and micro‐injection of purified fluorescently labeled CP was used to examine its nuclear uptake in Drosophila embryos. Both assays demonstrated that TYLCV CP is transported into plant‐and insect‐cell nuclei by an active process of nuclear import via a nuclear localization signal (NLS)‐specific pathway. Using the GUS assay and deletion analysis, the TYLCV CP NLS sequence was identified in the amino‐terminus of the protein. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Nuclear import of the capsid protein of tomato yellow leaf curl virus (TYLCV) in plant and insect cells

Loading next page...
 
/lp/wiley/nuclear-import-of-the-capsid-protein-of-tomato-yellow-leaf-curl-virus-y4l1rqDmcC
Publisher
Wiley
Copyright
Copyright © 1998 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
D.O.I.
10.1046/j.1365-313X.1998.00037.x
Publisher site
See Article on Publisher Site

Abstract

Summary The tomato yellow leaf curl virus (TYLCV) found in Israel is a whitefly‐transmitted monopartite geminivirus. Although geminiviruses have been found in the nuclei of phloem‐associated cells, the mechanism of viral invasion is poorly understood. The possible role of the TYLCV capsid protein (CP), the only known component of the viral coat, in virus transport into the host cell nucleus was investigated by monitoring its specific nuclear accumulation in plant and insect cells. CP was fused to the β‐glucuronidase (GUS) reporter enzyme to assay nuclear import in petunia protoplasts, and micro‐injection of purified fluorescently labeled CP was used to examine its nuclear uptake in Drosophila embryos. Both assays demonstrated that TYLCV CP is transported into plant‐and insect‐cell nuclei by an active process of nuclear import via a nuclear localization signal (NLS)‐specific pathway. Using the GUS assay and deletion analysis, the TYLCV CP NLS sequence was identified in the amino‐terminus of the protein.

Journal

The Plant JournalWiley

Published: Feb 1, 1998

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off