Neuroprotective role of monocarboxylate transport during glucose deprivation in slice cultures of rat hippocampus

Neuroprotective role of monocarboxylate transport during glucose deprivation in slice cultures of... 1 The effects of energy substrate removal and metabolic pathway block have been examined on neuronal and glial survival in organotypic slice cultures of rat hippocampus. 2 Slice cultures resisted 24 h of exogenous energy substrate deprivation. Application of 0.5 mM α‐cyano‐4‐hydroxycinnamate (4‐CIN) for 24 h resulted in specific damage to neuronal cell layers, which could be reversed by co‐application of 5 mM lactate. 3 Addition of 10 mM 2‐deoxyglucose in the absence of exogenous energy supply produced widespread cell death throughout the slice. This was partly reversed by co‐application of 5 mM lactate. 4 These effects of metabolic blockade on cell survival were qualitatively similar to the effects on population spikes recorded in the CA1 cell layer following 60 min application of these agents. 5 The data suggest that monocarboxylate trafficking from glia to neurons is an essential route for supply of energy substrates to neurons particularly when exogenous energy supply is restricted. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Physiology Wiley

Neuroprotective role of monocarboxylate transport during glucose deprivation in slice cultures of rat hippocampus

Loading next page...
 
/lp/wiley/neuroprotective-role-of-monocarboxylate-transport-during-glucose-QeYcJygmsC
Publisher
Wiley
Copyright
Copyright © 2001 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3751
eISSN
1469-7793
D.O.I.
10.1111/j.1469-7793.2001.0459i.x
Publisher site
See Article on Publisher Site

Abstract

1 The effects of energy substrate removal and metabolic pathway block have been examined on neuronal and glial survival in organotypic slice cultures of rat hippocampus. 2 Slice cultures resisted 24 h of exogenous energy substrate deprivation. Application of 0.5 mM α‐cyano‐4‐hydroxycinnamate (4‐CIN) for 24 h resulted in specific damage to neuronal cell layers, which could be reversed by co‐application of 5 mM lactate. 3 Addition of 10 mM 2‐deoxyglucose in the absence of exogenous energy supply produced widespread cell death throughout the slice. This was partly reversed by co‐application of 5 mM lactate. 4 These effects of metabolic blockade on cell survival were qualitatively similar to the effects on population spikes recorded in the CA1 cell layer following 60 min application of these agents. 5 The data suggest that monocarboxylate trafficking from glia to neurons is an essential route for supply of energy substrates to neurons particularly when exogenous energy supply is restricted.

Journal

The Journal of PhysiologyWiley

Published: Mar 1, 2001

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off