Neuronotrophic Factors Released by C6 Glioma Cells

Neuronotrophic Factors Released by C6 Glioma Cells Abstract: Glial cells have been shown previously to release factors that promote survival of central and peripheral neurons (neuronotrophic factors (NTFs)). We have investigated the release of NTFs by C6 cells, a rat glioma cell line, under different modes of conditioning. Media conditioned in the presence or absence of serum (C6 cell conditioned media (C6CMs)) were analyzed using biological, biochemical, and immunological assays. We report that (a) nuclear and cytoskeletal proteins were not present in C6CMs, indicating that C6CM proteins result from release by C6 cells rather than from cell death; (b) C6CM contained 1–3 μg protein/ml, corresponding to a secretion rate of about 0.5 pg protein per cell and day; (c) C6CM contained the neurite‐promoting factor laminin and low amounts of nerve growth factor; (d) the presence of fetal calf serum in the culture medium was essential for synthesis and release of NTFs; and (e) our C6CM contained at least three NTFs differing by their temporal secretory patterns and three NTFs differing by biochemical properties, indicating that C6 cells produce and secrete six different NTFs. Within these, nerve growth factor seems to be the only established NTF. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Neuronotrophic Factors Released by C6 Glioma Cells

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Publisher
Wiley
Copyright
Copyright © 1988 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1988.tb02474.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Glial cells have been shown previously to release factors that promote survival of central and peripheral neurons (neuronotrophic factors (NTFs)). We have investigated the release of NTFs by C6 cells, a rat glioma cell line, under different modes of conditioning. Media conditioned in the presence or absence of serum (C6 cell conditioned media (C6CMs)) were analyzed using biological, biochemical, and immunological assays. We report that (a) nuclear and cytoskeletal proteins were not present in C6CMs, indicating that C6CM proteins result from release by C6 cells rather than from cell death; (b) C6CM contained 1–3 μg protein/ml, corresponding to a secretion rate of about 0.5 pg protein per cell and day; (c) C6CM contained the neurite‐promoting factor laminin and low amounts of nerve growth factor; (d) the presence of fetal calf serum in the culture medium was essential for synthesis and release of NTFs; and (e) our C6CM contained at least three NTFs differing by their temporal secretory patterns and three NTFs differing by biochemical properties, indicating that C6 cells produce and secrete six different NTFs. Within these, nerve growth factor seems to be the only established NTF.

Journal

Journal of NeurochemistryWiley

Published: Jun 1, 1988

References

  • Purification of the chick eye ciliary neuronotrophic factor
    Barbin, Barbin; Manthorpe, Manthorpe; Varon, Varon
  • Neuroimmunology of gangliosides in human neurons and glial cells in culture
    Kim, Kim; Moretto, Moretto; Lee, Lee; Yu, Yu
  • Purification of a factor that promotes neurite outgrowth: isolation of laminin and associated molecules
    Lander, Lander; Fujii, Fujii; Reichardt, Reichardt
  • Neurite‐promoting factor in conditioned medium from RN22 schwannoma cultures. Bioassay, fractionation, and properties
    Manthorpe, Manthorpe; Varon, Varon; Adler, Adler
  • Laminin promotes neuritic regeneration from cultured peripheral and central neurons
    Manthorpe, Manthorpe; Engvall, Engvall; Ruoslahti, Ruoslahti; Longo, Longo; Davis, Davis; Varon, Varon
  • Secretion of nerve growth factor by central nervous system glioma cells in culture
    Murphy, Murphy; Oger, Oger; Saide, Saide; Blanchard, Blanchard; Arnason, Arnason; Hogan, Hogan; Pantazis, Pantazis; Young, Young
  • Criticism of the use of Coomassie Brilliant Blue G‐250 for the quantitative determinations of proteins
    Wilgenburg, Wilgenburg; Werkman, Werkman; Gorkom, Gorkom; Soons, Soons
  • An enzyme‐linked immunoassay for nerve growth factor (NGF): a tool for studying regulatory mechanisms involved in NGF production in brain and in peripheral tissues
    Weskamp, Weskamp; Otten, Otten

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