Nervous System‐Specific Proteins in Developing Rat Cerebral Cells in Culture

Nervous System‐Specific Proteins in Developing Rat Cerebral Cells in Culture Abstract: The nervous system‐specific proteins: synaptin, D1, D2, D3, glial fibrillary acidic protein (GFA) and 14‐3‐2, were quantified in dissociated cerebral cells from the foetal rat brain at various times of growth in culture. By approximately 1 week in culture, the neuronal membrane markers synaptin, D1, D2, and D3 could all be demonstrated. A maximum concentration of 10–20% for synaptin, D1, and D3 and 160% for D2, in comparison with the levels in adult forebrain, was attained during the 2nd week in vitro. The astroglial gliofilament marker GFA increased continuously, reaching by 38 days of cultivation an 18‐fold higher level than the concentration in adult forebrain. The neuronal cytoplasm marker 14‐3‐2 could be demonstrated in trace amounts, and only after more than 1 week in vitro. Neuronal cell bodies and processes stained by indirect immunofluorescence using an anti‐D2 serum were strongly fluorescent after 1 week in vitro. Immunofluorescence staining for GFA revealed a cytoplasmatic filamentous network in perinuclear areas and processes of, presumably, astroblasts. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Nervous System‐Specific Proteins in Developing Rat Cerebral Cells in Culture

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Publisher
Wiley
Copyright
Copyright © 1980 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1980.tb09001.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: The nervous system‐specific proteins: synaptin, D1, D2, D3, glial fibrillary acidic protein (GFA) and 14‐3‐2, were quantified in dissociated cerebral cells from the foetal rat brain at various times of growth in culture. By approximately 1 week in culture, the neuronal membrane markers synaptin, D1, D2, and D3 could all be demonstrated. A maximum concentration of 10–20% for synaptin, D1, and D3 and 160% for D2, in comparison with the levels in adult forebrain, was attained during the 2nd week in vitro. The astroglial gliofilament marker GFA increased continuously, reaching by 38 days of cultivation an 18‐fold higher level than the concentration in adult forebrain. The neuronal cytoplasm marker 14‐3‐2 could be demonstrated in trace amounts, and only after more than 1 week in vitro. Neuronal cell bodies and processes stained by indirect immunofluorescence using an anti‐D2 serum were strongly fluorescent after 1 week in vitro. Immunofluorescence staining for GFA revealed a cytoplasmatic filamentous network in perinuclear areas and processes of, presumably, astroblasts.

Journal

Journal of NeurochemistryWiley

Published: Dec 1, 1980

References

  • Regional distribution of the synaptic membrane proteins: Synaptin, D1, D2, and D3
    Bock, Bock; Braestrup, Braestrup
  • Determination of brain specific antigens in short term cultivated rat astroglial cells and in rat synaptosomes
    Bock, Bock; Jørgensen, Jørgensen; Dittmann, Dittmann; Eng, Eng
  • Brain specific synaptosomal membrane proteins demonstrated by crossed immunoelectrophoresis
    Jørgensen, Jørgensen; Bock, Bock
  • Protein profiles of rat embryo cerebral cells during differentiation in culture
    Richter‐Landsberg, Richter‐Landsberg; Yavin, Yavin

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