Nasal immunization of mice with Lactobacillus casei expressing the pneumococcal surface protein C primes the immune system and decreases pneumococcal nasopharyngeal colonization in mice

Nasal immunization of mice with Lactobacillus casei expressing the pneumococcal surface protein C... Streptococcus pneumoniae colonizes the upper respiratory tract of healthy individuals, from where it can be transmitted to the community. Occasionally, bacteria invade sterile niches, causing diseases. The pneumococcal surface protein C (PspC) is a virulence factor that is important during colonization and the systemic phases of the diseases. Here, we have evaluated the effect of nasal or sublingual immunization of mice with Lactobacillus casei expressing PspC, as well as prime‐boosting protocols using recombinant PspC, on nasopharyngeal pneumococcal colonization. None of the protocols tested was able to elicit significant levels of anti‐PspC antibodies before challenge. However, a significant decrease in pneumococcal recovery from the nasopharynx was observed in animals immunized through the nasal route with L. casei‐PspC. Immune responses evaluated after colonization challenge in this group of mice were characterized by an increase in mucosal anti‐PspC immunoglobulin A (IgA) 5 days later, a time point in which the pneumococcal loads were already low. A negative correlation between the concentrations of anti‐PspC IgA and pneumococcal recovery from the nasopharynx was observed, with animals with the lowest colonization levels having higher IgA concentrations. These results show that nasal immunization with L. casei‐PspC primes the immune system of mice, prompting faster immune responses that result in a decrease in pneumococcal colonization. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pathogens and Disease Wiley

Nasal immunization of mice with Lactobacillus casei expressing the pneumococcal surface protein C primes the immune system and decreases pneumococcal nasopharyngeal colonization in mice

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Publisher
Wiley
Copyright
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
ISSN
0928-8244
eISSN
2049-632X
D.O.I.
10.1111/j.1574-695X.2011.00809.x
Publisher site
See Article on Publisher Site

Abstract

Streptococcus pneumoniae colonizes the upper respiratory tract of healthy individuals, from where it can be transmitted to the community. Occasionally, bacteria invade sterile niches, causing diseases. The pneumococcal surface protein C (PspC) is a virulence factor that is important during colonization and the systemic phases of the diseases. Here, we have evaluated the effect of nasal or sublingual immunization of mice with Lactobacillus casei expressing PspC, as well as prime‐boosting protocols using recombinant PspC, on nasopharyngeal pneumococcal colonization. None of the protocols tested was able to elicit significant levels of anti‐PspC antibodies before challenge. However, a significant decrease in pneumococcal recovery from the nasopharynx was observed in animals immunized through the nasal route with L. casei‐PspC. Immune responses evaluated after colonization challenge in this group of mice were characterized by an increase in mucosal anti‐PspC immunoglobulin A (IgA) 5 days later, a time point in which the pneumococcal loads were already low. A negative correlation between the concentrations of anti‐PspC IgA and pneumococcal recovery from the nasopharynx was observed, with animals with the lowest colonization levels having higher IgA concentrations. These results show that nasal immunization with L. casei‐PspC primes the immune system of mice, prompting faster immune responses that result in a decrease in pneumococcal colonization.

Journal

Pathogens and DiseaseWiley

Published: Aug 1, 2011

References

  • Nasal immunization of mice with Lactobacillus casei expressing the Pneumococcal Surface Protein A
    Campos, Campos; Darrieux, Darrieux; Ferreira, Ferreira
  • Protective effect of intranasally inoculated Lactobacillus fermentum against Streptococcus pneumoniae challenge on the mouse respiratory tract
    Cangemi de Gutierrez, Cangemi de Gutierrez; Santos, Santos; Nader‐Macias, Nader‐Macias
  • Microbes versus microbes
    Cross, Cross
  • Immunization of mice with Lactobacillus casei expressing intimin fragments produces antibodies able to inhibit the adhesion of enteropathogenic Escherichia coli to cultivated epithelial cells
    Ferreira, Ferreira; Campos, Campos; Abe, Abe; Trabulsi, Trabulsi; Elias, Elias; Ho, Ho; Oliveira, Oliveira
  • Allelic variation in the highly polymorphic locus pspC of Streptococcus pneumoniae
    Iannelli, Iannelli; Oggioni, Oggioni; Pozzi, Pozzi
  • Adjuvant properties and colonization potential of adhering and non‐adhering Lactobacillus spp. following oral administration to mice
    Plant, Plant; Conway, Conway

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