Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Myelin‐phagocytosing macrophages in isolated sciatic and optic nerves reveal a unique reactive phenotype

Myelin‐phagocytosing macrophages in isolated sciatic and optic nerves reveal a unique reactive... Macrophages are key effectors in demyelinating diseases of the central and peripheral nervous system by phagocytosing myelin and releasing immunoregulatory mediators. Here, we report on a distinct, a priori anti‐inflammatory reaction of macrophages phagocytosing myelin upon contact with damaged nerve tissue. Macrophages rapidly invaded peripheral (sciatic) and central (optic) nerve tissues in vitro, readily incorporated myelin and expressed high levels of phagocytosis‐associated molecules (e.g., Fc and scavenger receptors). In contrast, factors involved in antigen presentation (MHC class‐II, CD80, CD86) revealed only a restricted expression. In parallel, a highly ordered appearance of cytokines and chemokines was detected. IL‐10, IL‐6, CCL22, and CXCL1 were immediately but transiently induced, whereas CCL2, CCL11, and TGFβ revealed more persisting levels. Such a profile would attract neutrophils, monocytes/macrophages, and Th2 cells as well as bias for a Th2‐supporting environment. Importantly, proinflammatory/Th1‐supporting factors, such as TNFα, IL‐12p70, CCL3, and CCL5, were not induced. Still the simultaneous presence of TGFβ and IL‐6 could assist Th17 development, further depending on yet not present IL‐23. The release pattern was clearly distinct from reactive phenotypes induced in isolated macrophages and microglia upon treatment with IL‐4, IL‐13, bacterial lipopolysaccharide, IFNγ, or purified myelin. Nerve‐exposed macrophages thus commit to a unique functional orientation. © 2007 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Glia Wiley

Myelin‐phagocytosing macrophages in isolated sciatic and optic nerves reveal a unique reactive phenotype

Loading next page...
 
/lp/wiley/myelin-phagocytosing-macrophages-in-isolated-sciatic-and-optic-nerves-fMmCU1Yy3Q
Publisher
Wiley
Copyright
Copyright © 2007 Wiley‐Liss, Inc.
ISSN
0894-1491
eISSN
1098-1136
DOI
10.1002/glia.20611
pmid
18069669
Publisher site
See Article on Publisher Site

Abstract

Macrophages are key effectors in demyelinating diseases of the central and peripheral nervous system by phagocytosing myelin and releasing immunoregulatory mediators. Here, we report on a distinct, a priori anti‐inflammatory reaction of macrophages phagocytosing myelin upon contact with damaged nerve tissue. Macrophages rapidly invaded peripheral (sciatic) and central (optic) nerve tissues in vitro, readily incorporated myelin and expressed high levels of phagocytosis‐associated molecules (e.g., Fc and scavenger receptors). In contrast, factors involved in antigen presentation (MHC class‐II, CD80, CD86) revealed only a restricted expression. In parallel, a highly ordered appearance of cytokines and chemokines was detected. IL‐10, IL‐6, CCL22, and CXCL1 were immediately but transiently induced, whereas CCL2, CCL11, and TGFβ revealed more persisting levels. Such a profile would attract neutrophils, monocytes/macrophages, and Th2 cells as well as bias for a Th2‐supporting environment. Importantly, proinflammatory/Th1‐supporting factors, such as TNFα, IL‐12p70, CCL3, and CCL5, were not induced. Still the simultaneous presence of TGFβ and IL‐6 could assist Th17 development, further depending on yet not present IL‐23. The release pattern was clearly distinct from reactive phenotypes induced in isolated macrophages and microglia upon treatment with IL‐4, IL‐13, bacterial lipopolysaccharide, IFNγ, or purified myelin. Nerve‐exposed macrophages thus commit to a unique functional orientation. © 2007 Wiley‐Liss, Inc.

Journal

GliaWiley

Published: Feb 1, 2008

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$499/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month