Myelin basic protein and transferrin characterize different subpopulations of oligodendrocytes in rat primary glial cultures

Myelin basic protein and transferrin characterize different subpopulations of oligodendrocytes in... The iron transport glycoprotein, transferrin (Tf), localizes exclusively in oligodendrocytes in brain tissue sections. Previously, we showed that Tf is also expressed in oligodendrocytes in primary cultures established from newborn rat brains. Its developmental appearance precedes that of galactocerebroside (GC). In this study, Tf expression in primary brain cell cultures was investigated over a 4‐week period in relation to GC and myelin basic protein (MBP), respectively, early and late markers of oligodendrocyte development. From 9 days in vitro and thereafter, all Tf+ cells were also found to be GC+. With increasing age the number of Tf+ cells decreased while the number of MBP+ cells increased. However, less than 10% of oligodendrocytes co‐expressed Tf and MBP at any age. MBP+ cells were largely found in cell clusters which increased in size and number with age in culture. Interestingly, Tf+ cells were located around the clusters of MBP+ cells which displayed elaborate branched processes. The transient expression of Tf in oligodendrocytes which become MBP+, suggests a role for Tf in the early stages of myelinogenesis. The results also demonstrate the existence of three phenotypically distinct populations of oligodendrocytes. A new model of developmental and functional subpopulations of oligodendrocytes is proposed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

Myelin basic protein and transferrin characterize different subpopulations of oligodendrocytes in rat primary glial cultures

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Publisher
Wiley
Copyright
Copyright © 1988 Alan R. Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
D.O.I.
10.1002/jnr.490210210
Publisher site
See Article on Publisher Site

Abstract

The iron transport glycoprotein, transferrin (Tf), localizes exclusively in oligodendrocytes in brain tissue sections. Previously, we showed that Tf is also expressed in oligodendrocytes in primary cultures established from newborn rat brains. Its developmental appearance precedes that of galactocerebroside (GC). In this study, Tf expression in primary brain cell cultures was investigated over a 4‐week period in relation to GC and myelin basic protein (MBP), respectively, early and late markers of oligodendrocyte development. From 9 days in vitro and thereafter, all Tf+ cells were also found to be GC+. With increasing age the number of Tf+ cells decreased while the number of MBP+ cells increased. However, less than 10% of oligodendrocytes co‐expressed Tf and MBP at any age. MBP+ cells were largely found in cell clusters which increased in size and number with age in culture. Interestingly, Tf+ cells were located around the clusters of MBP+ cells which displayed elaborate branched processes. The transient expression of Tf in oligodendrocytes which become MBP+, suggests a role for Tf in the early stages of myelinogenesis. The results also demonstrate the existence of three phenotypically distinct populations of oligodendrocytes. A new model of developmental and functional subpopulations of oligodendrocytes is proposed.

Journal

Journal of Neuroscience ResearchWiley

Published: Oct 1, 1988

References

  • Development of transferrin‐positive oligodendrocytes in the rat central nervous system
    Connor, Connor; Fine, Fine
  • Myelin specific proteins and glycolipids in rat Schwann cells and oligodendrocytes in culture
    Mirsky, Mirsky; Wentner, Wentner; Abney, Abney; Pruss, Pruss; Gavrilovick, Gavrilovick; Raff, Raff
  • Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue
    McCarthy, McCarthy; de Vellis, de Vellis

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