Multiplex PCR scheme for variant plasmid mediated class C β‐lactamase typing

Multiplex PCR scheme for variant plasmid mediated class C β‐lactamase typing INTRODUCTIONEnterobacteriaceae are the most common pathogens causing community‐ and hospital‐acquired infections and the antibiotic resistance Enterobacteriaceae have been reported worldwide. The infection tends to exert hospital burden for both health care institution and patient. The major driving force of antibiotic resistance within this family is the production of broad spectrum β‐lactamases including extended spectrum β‐lactamases (ESBLs), plasmid mediated AmpC cephalosporinases (pAmpC), and carbapenemases. Furthermore, extended spectrum cephalosporin resistance (ESC‐R) Enterobacteriaceae phenotype may harbor one or many types of broad spectrum β‐lactamases and may also harbor multiple β‐lactamase genes of the same classes of β‐lactamases in one organism. Because of these β‐lactamase genes are mediated on plasmid, the resistance genes were easily transferred to other bacterial cells, species, and genus. These results inferred to the increasing rates of ESC‐R Enterobacteriaceae.Plasmid‐mediated AmpC β‐lactamase genes are descended from the chromosomal ampC genes of several organisms such as Citrobacter fruendii, Enterobacter cloacae, Hafnia alvei, and Morganella morganii. Because of lack association with regulation genes (ampR), pAmpC is usually overexpression. Although less common than ESBLs, plasmid AmpC β‐lactamases (pAmpC) have been reported worldwide with various prevalence rates. These enzymes confer resistance to penicillins, first to third generation cephalosporins, cephamycins, monobactams, and also poorly inhibited http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Laboratory Analysis Wiley

Multiplex PCR scheme for variant plasmid mediated class C β‐lactamase typing

Loading next page...
 
/lp/wiley/multiplex-pcr-scheme-for-variant-plasmid-mediated-class-c-lactamase-p8ryZYGmHD
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0887-8013
eISSN
1098-2825
D.O.I.
10.1002/jcla.22298
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONEnterobacteriaceae are the most common pathogens causing community‐ and hospital‐acquired infections and the antibiotic resistance Enterobacteriaceae have been reported worldwide. The infection tends to exert hospital burden for both health care institution and patient. The major driving force of antibiotic resistance within this family is the production of broad spectrum β‐lactamases including extended spectrum β‐lactamases (ESBLs), plasmid mediated AmpC cephalosporinases (pAmpC), and carbapenemases. Furthermore, extended spectrum cephalosporin resistance (ESC‐R) Enterobacteriaceae phenotype may harbor one or many types of broad spectrum β‐lactamases and may also harbor multiple β‐lactamase genes of the same classes of β‐lactamases in one organism. Because of these β‐lactamase genes are mediated on plasmid, the resistance genes were easily transferred to other bacterial cells, species, and genus. These results inferred to the increasing rates of ESC‐R Enterobacteriaceae.Plasmid‐mediated AmpC β‐lactamase genes are descended from the chromosomal ampC genes of several organisms such as Citrobacter fruendii, Enterobacter cloacae, Hafnia alvei, and Morganella morganii. Because of lack association with regulation genes (ampR), pAmpC is usually overexpression. Although less common than ESBLs, plasmid AmpC β‐lactamases (pAmpC) have been reported worldwide with various prevalence rates. These enzymes confer resistance to penicillins, first to third generation cephalosporins, cephamycins, monobactams, and also poorly inhibited

Journal

Journal of Clinical Laboratory AnalysisWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial