Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Suszkiw, R. Beach, G. Pilar (1976)
CHOLINE UPTAKE BY CHOLINERGIC NEURON CELL SOMASJournal of Neurochemistry, 26
J. Rossier (1976)
PURIFICATION OF RAT BRAIN CHOLINE ACETYLTRANSFERASE 1Journal of Neurochemistry, 26
O. Lowry, N. Rosebrough, A. Farr, R. Randall (1951)
Protein measurement with the Folin phenol reagent.The Journal of biological chemistry, 193 1
H. Gutfreund (1975)
Enzyme kineticsNature, 258
L. Potter, W. Murphy (1967)
Electrophoresis of acetylcholinc, choline and related compoundsBiochemical Pharmacology, 16
(1976)
Biochim . biophys
(1977)
IUAPC-IUB Commission on Biochemical Nomenclature 61. 111-112. 62, 398. Ther. 192, 8694
R. Massarelli, M. Sensenbrenner, A. Ebel, P. Mandel (1974)
Kinetics of choline uptake in mixed neuronal-glial, and exclusively glial cultures.Neurobiology, 4 6
C. Atterwill, A. Prince (1977)
PROCEEDINGS OF THE British Pharmacological SocietyBritish Journal of Pharmacology, 61
A. Latner (1968)
CHAPTER VII – Ontogenic and Phylogenic Studies
L. Barker, T. Mittag (1975)
Comparative studies of substrates and inhibitors of choline transport and choline acetyltransferase.The Journal of pharmacology and experimental therapeutics, 192 1
L. Barker, M. Dowdall, T. Mittag (1975)
Comparative studies on synaptosomes: high-affinity uptake and acetylation of N-[Me-3H]choline and N-[Me-3H]N-hydroxyethylpyrrolidiniumBrain Research, 86
G. Wilkinson (1961)
Statistical estimations in enzyme kinetics.The Biochemical journal, 80
Rosemary Polsky, Louis Shuster (1976)
Preparation and characterization of two isozymes of choline acetyltransferase from squid head ganglia.Biochimica et biophysica acta, 445 1
P. Shea, M. Aprison (1977)
THE DISTRIBUTION OF ACETYL‐CoA IN SPECIFIC AREAS OF THE CNS OF THE RAT AS MEASURED BY A MODIFICATION OF A RADIO‐ENZYMATIC ASSAY FOR ACETYLCHOLINE AND CHOLINE 1Journal of Neurochemistry, 28
J. Coyle, S. Enna (1976)
Neurochemical aspects of the ontogenesis of gabanergic neurons in the rat brainBrain Research, 111
H. Yamamura, S. Snyder (1973)
HIGH AFFINITY TRANSPORT OF CHOLINE INTO SYNAPTOSOMES OF RAT BRAIN 1Journal of Neurochemistry, 21
R. Massarelli, P. Syapin, E. Noble (1976)
Increased uptake of choline by neural cell cultures chronically exposed to ethanol.Life sciences, 18 4
M. Sorimachi, K. Kataoka (1975)
High affinity choline uptake: An early index of cholinergic innervation in rat brainBrain Research, 94
M. Toru, M. Aprison (1966)
BRAIN ACETYLCHOLINE STUDIES: A NEW EXTRACTION PROCEDURE *Journal of Neurochemistry, 13
Vijendra Singh, E. Mcgeer (1977)
Choline acetyltransferase in developing rat brain and spinal cordBrain Research, 127
J. Suszkiw, G. Pilar (1976)
SELECTIVE LOCALIZATION OF A HIGH AFFINITY CHOLINE UPTAKE SYSTEM AND ITS ROLE IN ACh FORMATTON IN CHOLINERGIC NERVE TERMINALSJournal of Neurochemistry, 26
F. Fonnum, D. Malthe-Sørenssen (1973)
MEMBRANE AFFINITIES AND SUBCELLULAR DISTRIBUTION OF THE DIFFERENT MOLECULAR FORMS OF CHOLINE ACETYLTRANSFERASE FROM RATJournal of Neurochemistry, 20
F. Fonnum (1975)
A rapid radiochemical method for the determination of choline acetyltransferaseJournal of Neurochemistry, 24
(1968)
Isoenzymes in Biology and Medicine pp, 119-127
(1974)
Neurobiolugy 4 , 414418
D. Malthe-Sørenssen, F. Fonnum (1972)
Multiple forms of choline acetyltransferase in several species demonstrated by isoelectric focusing.The Biochemical journal, 127 1
Jay Simon, Samir Atweh, Michael Kuhar (1976)
SODIUM‐DEPENDENT HIGH AFFINITY CHOLINE UPTAKE: A REGULATORY STEP IN THE SYNTHESIS OF ACETYLCHOLINEJournal of Neurochemistry, 26
M. Neal, J. Gilroy (1975)
High-affinity choline transport in the isolated retinaBrain Research, 93
M. Kuhar, V. Sethy, R. Roth, G. Aghajanian (1973)
CHOLINE: SELECTIVE ACCUMULATION BY CENTRAL CHOLINERGIC NEURONSJournal of Neurochemistry, 20
F. Fonnum, D. Malthe-Sørenssen (1972)
Molecular properties of choline acetyltransferase and their importance for the compartmentation of acetylcholine synthesis.Progress in brain research, 36
Abstract— The multiple molecular forms of choline acetyltransferase (ChAT) were analysed during the postnatal development of rat brain. Changes in the sodium‐dependent, high affinity uptake of (3H)choline (HAUC) and in the efficiency of conversion of labelled choline into ACh in vitro were also examined. Both mature and 7‐day old brain contained three molecular forms of ChAT, with isoelectric points of pH 7.3, 7.9 and 8.3, but the immature brain appeared to contain smaller concentrations of the most basic form of the enzyme (pI = 8.3). Of the total choline uptake measured in slices of frontal cortex, adult samples exhibited a greater proportion of HAUC than 7‐day samples and appeared to acetylate more efficiently the (3H)choline accumulated by high affinity uptake. This evidence suggests a basic molecular form of ChAT, appearing in rat brain during postnatal development, might be responsible for the efficient coupling of the high affinity uptake and subsequent acetylation of choline in cholinergic nerve terminals.
Journal of Neurochemistry – Wiley
Published: Sep 1, 1978
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.