Monoclonal Antibodies to Hepatitis B Surface Antigen (HBsAg) as a Tool for the Automated HBsAg Screening

Monoclonal Antibodies to Hepatitis B Surface Antigen (HBsAg) as a Tool for the Automated HBsAg... Abstract. Hybridomas secreting monoclonal antibodies to hepatitis B surface antigen (HBsAg) were established by fusion of the spleen cells from mice immunized with purified HBsAg with the mouse myeloma cell line P3‐NSI/l‐Ag4‐l. The monoclonal antibodies to the group‐specific antigen (a) produced by one of them were used for the automated screening of HBsAg on the Groupamatic 360 (Kontron International). Its sensitivity is almost equal, but slightly inferior, to the system employing polyclonal horse antibodies to HBsAg; it barely detects 6 ng/ml of HBsAg. It is also as highly specific as the system with polyclonal antibodies; the incidence of false‐positive reactions is 0.2%. These results indicate that the monoclonal antibodies will become a practical source for the HBsAg screening on the Groupamatic. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Vox Sanguinis Wiley

Monoclonal Antibodies to Hepatitis B Surface Antigen (HBsAg) as a Tool for the Automated HBsAg Screening

Vox Sanguinis, Volume 45 (2) – Aug 1, 1983

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Publisher
Wiley
Copyright
© 1983 S. Karger AG, Basel
ISSN
0042-9007
eISSN
1423-0410
DOI
10.1111/j.1423-0410.1983.tb01894.x
Publisher site
See Article on Publisher Site

Abstract

Abstract. Hybridomas secreting monoclonal antibodies to hepatitis B surface antigen (HBsAg) were established by fusion of the spleen cells from mice immunized with purified HBsAg with the mouse myeloma cell line P3‐NSI/l‐Ag4‐l. The monoclonal antibodies to the group‐specific antigen (a) produced by one of them were used for the automated screening of HBsAg on the Groupamatic 360 (Kontron International). Its sensitivity is almost equal, but slightly inferior, to the system employing polyclonal horse antibodies to HBsAg; it barely detects 6 ng/ml of HBsAg. It is also as highly specific as the system with polyclonal antibodies; the incidence of false‐positive reactions is 0.2%. These results indicate that the monoclonal antibodies will become a practical source for the HBsAg screening on the Groupamatic.

Journal

Vox SanguinisWiley

Published: Aug 1, 1983

References

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