Monitoring ovarian function in marmosets and tamarins by the measurement of urinary estrogen metabolites

Monitoring ovarian function in marmosets and tamarins by the measurement of urinary estrogen... Practical aspects of urinary estrogen analysis were considered with regard to establishing simple and reliable methods for monitoring ovarian function in marmosets and tamarins. Changes in the hormone:creatinine ratio in small volumes of urine from the common marmoset were significantly correlated with changes in 24‐h excretion. Comparison of the metabolism and excretion of estrogens during the ovarian cycle in the common marmoset and cottontop tamarin revealed interesting species differences. High concentrations of conjugated estrone were measured in marmoset plasma, but estradiol 17β was the predominant estrogen in urine. In contrast, estrone was the most abundant estrogen measured in tamarin urine. Both species excreted very little estriol. Sulfates and glucuronides were present in urine in similar proportions before ovulation in the marmoset, although after ovulation sulfates were the more abundant. Conversely, most of the estrogens in tamarin urine appeared to be conjugated as glucuronides. Direct assay for estrone sulfate was applied to the measurement of urinary estrogen excretion during the ovarian cycle in a marmoset. The results compared well with those for total estradiol 17β after hydrolysis and ether extraction. The use of direct assays for conjugated estrogens in small volumes of urine is suggested as a practical method for monitoring ovarian function in marmosets and tamarins. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Primatology Wiley

Monitoring ovarian function in marmosets and tamarins by the measurement of urinary estrogen metabolites

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Publisher
Wiley
Copyright
Copyright © 1984 Wiley‐Liss, Inc., A Wiley Company
ISSN
0275-2565
eISSN
1098-2345
DOI
10.1002/ajp.1350060306
Publisher site
See Article on Publisher Site

Abstract

Practical aspects of urinary estrogen analysis were considered with regard to establishing simple and reliable methods for monitoring ovarian function in marmosets and tamarins. Changes in the hormone:creatinine ratio in small volumes of urine from the common marmoset were significantly correlated with changes in 24‐h excretion. Comparison of the metabolism and excretion of estrogens during the ovarian cycle in the common marmoset and cottontop tamarin revealed interesting species differences. High concentrations of conjugated estrone were measured in marmoset plasma, but estradiol 17β was the predominant estrogen in urine. In contrast, estrone was the most abundant estrogen measured in tamarin urine. Both species excreted very little estriol. Sulfates and glucuronides were present in urine in similar proportions before ovulation in the marmoset, although after ovulation sulfates were the more abundant. Conversely, most of the estrogens in tamarin urine appeared to be conjugated as glucuronides. Direct assay for estrone sulfate was applied to the measurement of urinary estrogen excretion during the ovarian cycle in a marmoset. The results compared well with those for total estradiol 17β after hydrolysis and ether extraction. The use of direct assays for conjugated estrogens in small volumes of urine is suggested as a practical method for monitoring ovarian function in marmosets and tamarins.

Journal

American Journal of PrimatologyWiley

Published: Jan 1, 1984

References

  • The relationship between plasma progesterone and the timing of ovulation and early embryonic development in the marmoset monkey
    Harlow, Harlow; Gems, Gems; Hodges, Hodges; Hearn, Hearn
  • Urinary immunoreactive estrogen and pregnanediol‐3‐glucuronide during the normal menstrual cycle of the female lowland gorilla ( Gorilla gorilla )
    Mitchell, Mitchell; Presley, Presley; Czekala, Czekala; Lasley, Lasley

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