Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the Iberian Peninsula

Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the... Random amplified polymorphic DNA (RAPD) analysis has been used to identify the eight Barbus species of the Iberian Peninsula, whose morphological differentiation is difficult. Ten random primers were employed to generate RAPD markers. Different RAPD profiles were observed for the different species. Four species‐specific markers were found in B. bocagei, seven in B. comizo, five in B. graellsii, three in B. guiraonis, eight in B. haasi, 13 in B. meridionalis, four in B. microcephalus and four in B. sclateri. Evidence is presented that RAPD markers constitute useful tools for accurate taxonomic identification of Spanish barbels which is one of the first prerequisites in effective conservation programmes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Fish Biology Wiley

Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the Iberian Peninsula

Journal of Fish Biology, Volume 59 (6) – Dec 1, 2001

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Publisher
Wiley
Copyright
Copyright © 2001 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-1112
eISSN
1095-8649
D.O.I.
10.1111/j.1095-8649.2001.tb00223.x
Publisher site
See Article on Publisher Site

Abstract

Random amplified polymorphic DNA (RAPD) analysis has been used to identify the eight Barbus species of the Iberian Peninsula, whose morphological differentiation is difficult. Ten random primers were employed to generate RAPD markers. Different RAPD profiles were observed for the different species. Four species‐specific markers were found in B. bocagei, seven in B. comizo, five in B. graellsii, three in B. guiraonis, eight in B. haasi, 13 in B. meridionalis, four in B. microcephalus and four in B. sclateri. Evidence is presented that RAPD markers constitute useful tools for accurate taxonomic identification of Spanish barbels which is one of the first prerequisites in effective conservation programmes.

Journal

Journal of Fish BiologyWiley

Published: Dec 1, 2001

References

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