Molecular characterization and gene silencing of Laccase 1 in the grain aphid, Sitobion avenae

Molecular characterization and gene silencing of Laccase 1 in the grain aphid, Sitobion avenae Laccase 1 (Lac1), a polyphenol oxidase, has been proposed to be involved in insect iron metabolism and immunity responses. However, little information is available on the roles of Lac 1 in insect–plant interactions. The grain aphid Sitobion avenae is one of the most destructive pests of cereal, directly drawing phloem sap and transmitting viruses. In the present study, we first cloned the open reading frame (ORF) of Lac 1 from S. avenae, and the putative protein sequence was predicted to have a carboxyl‐terminal transmembrane domain. We found that SaLac1 had higher expression levels in the fourth and adult stages using reverse transcription real‐time quantitative PCR (RT‐qPCR). SaLac 1 was highly expressed in the salivary gland and midgut and also in wingless compared with winged morphs. After feeding on aphid‐resistant wheat with a high total phenol content, the expression level of SaLac 1 increased significantly. RNA interference (RNAi) by oral feeding successfully inhibited the transcript levels of SaLac 1, and the knockdown of Lac 1 significantly decreased the survival rate of S. avenae on aphid‐resistant wheat. Our study demonstrated that S. avenae Lac1 was involved in the detoxification of phenolic compounds in wheat and was essential for the aphid to adapt to resistant plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Insect Biochemistry and Physiology Wiley

Molecular characterization and gene silencing of Laccase 1 in the grain aphid, Sitobion avenae

Loading next page...
 
/lp/wiley/molecular-characterization-and-gene-silencing-of-laccase-1-in-the-9n0TyfIj0q
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0739-4462
eISSN
1520-6327
D.O.I.
10.1002/arch.21446
Publisher site
See Article on Publisher Site

Abstract

Laccase 1 (Lac1), a polyphenol oxidase, has been proposed to be involved in insect iron metabolism and immunity responses. However, little information is available on the roles of Lac 1 in insect–plant interactions. The grain aphid Sitobion avenae is one of the most destructive pests of cereal, directly drawing phloem sap and transmitting viruses. In the present study, we first cloned the open reading frame (ORF) of Lac 1 from S. avenae, and the putative protein sequence was predicted to have a carboxyl‐terminal transmembrane domain. We found that SaLac1 had higher expression levels in the fourth and adult stages using reverse transcription real‐time quantitative PCR (RT‐qPCR). SaLac 1 was highly expressed in the salivary gland and midgut and also in wingless compared with winged morphs. After feeding on aphid‐resistant wheat with a high total phenol content, the expression level of SaLac 1 increased significantly. RNA interference (RNAi) by oral feeding successfully inhibited the transcript levels of SaLac 1, and the knockdown of Lac 1 significantly decreased the survival rate of S. avenae on aphid‐resistant wheat. Our study demonstrated that S. avenae Lac1 was involved in the detoxification of phenolic compounds in wheat and was essential for the aphid to adapt to resistant plants.

Journal

Archives of Insect Biochemistry and PhysiologyWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off