Molecular characterization and gene silencing of Laccase 1 in the grain aphid, Sitobion avenae

Molecular characterization and gene silencing of Laccase 1 in the grain aphid, Sitobion avenae Laccase 1 (Lac1), a polyphenol oxidase, has been proposed to be involved in insect iron metabolism and immunity responses. However, little information is available on the roles of Lac 1 in insect–plant interactions. The grain aphid Sitobion avenae is one of the most destructive pests of cereal, directly drawing phloem sap and transmitting viruses. In the present study, we first cloned the open reading frame (ORF) of Lac 1 from S. avenae, and the putative protein sequence was predicted to have a carboxyl‐terminal transmembrane domain. We found that SaLac1 had higher expression levels in the fourth and adult stages using reverse transcription real‐time quantitative PCR (RT‐qPCR). SaLac 1 was highly expressed in the salivary gland and midgut and also in wingless compared with winged morphs. After feeding on aphid‐resistant wheat with a high total phenol content, the expression level of SaLac 1 increased significantly. RNA interference (RNAi) by oral feeding successfully inhibited the transcript levels of SaLac 1, and the knockdown of Lac 1 significantly decreased the survival rate of S. avenae on aphid‐resistant wheat. Our study demonstrated that S. avenae Lac1 was involved in the detoxification of phenolic compounds in wheat and was essential for the aphid to adapt to resistant plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Insect Biochemistry and Physiology Wiley

Molecular characterization and gene silencing of Laccase 1 in the grain aphid, Sitobion avenae

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0739-4462
eISSN
1520-6327
D.O.I.
10.1002/arch.21446
Publisher site
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Abstract

Laccase 1 (Lac1), a polyphenol oxidase, has been proposed to be involved in insect iron metabolism and immunity responses. However, little information is available on the roles of Lac 1 in insect–plant interactions. The grain aphid Sitobion avenae is one of the most destructive pests of cereal, directly drawing phloem sap and transmitting viruses. In the present study, we first cloned the open reading frame (ORF) of Lac 1 from S. avenae, and the putative protein sequence was predicted to have a carboxyl‐terminal transmembrane domain. We found that SaLac1 had higher expression levels in the fourth and adult stages using reverse transcription real‐time quantitative PCR (RT‐qPCR). SaLac 1 was highly expressed in the salivary gland and midgut and also in wingless compared with winged morphs. After feeding on aphid‐resistant wheat with a high total phenol content, the expression level of SaLac 1 increased significantly. RNA interference (RNAi) by oral feeding successfully inhibited the transcript levels of SaLac 1, and the knockdown of Lac 1 significantly decreased the survival rate of S. avenae on aphid‐resistant wheat. Our study demonstrated that S. avenae Lac1 was involved in the detoxification of phenolic compounds in wheat and was essential for the aphid to adapt to resistant plants.

Journal

Archives of Insect Biochemistry and PhysiologyWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

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