Mode of antinociceptive action of flupirtine in the rat

Mode of antinociceptive action of flupirtine in the rat 1 Flupirtine is a novel, centrally acting, non‐opioid analgesic agent. The present investigation was undertaken to ascertain which neuronal systems might be responsible for its antinociceptive effect in rodents. The antinociceptive responses to the test compounds were examined in the tail‐flick test. 2 The selective destruction of noradrenergic pathways by 6‐hydroxydopamine considerably reduced the flupirtine‐induced inhibition of nociceptive responses but not the clonidine‐induced antinociception which was significantly enhanced. Depletion of spinal 5‐hydroxytryptaminergic pathways by pretreatment with 5,7‐dihydroxytryptamine failed to affect the action of flupirtine and clonidine. 3 The depletion of neurotransmitters by reserpine totally abolished the antinociceptive action of flupirtine. By contrast, clonidine‐induced inhibition of nociceptive responses remained unchanged. 4 Inhibition of the synthesis of noradrenaline by α‐methyl‐L‐p‐tyrosine attenuated the antinociception induced by flupirtine. In contrast, inhibition of the synthesis of 5‐hydroxytryptamine by (±)−6‐fluorotryptophan did not influence the antinociceptive activity of flupirtine. 5 Inhibition of noradrenaline uptake by imipramine led to a significant augmentation of flupirtine‐induced antinociception. 6 Selective antagonists at α‐adrenoceptors significantly decreased the antinociceptive action of flupirtine. Antinociception induced by clonidine was significantly diminished by idazoxan but not by prazosin. 7 The 5‐hydroxytryptamine (5‐HT) antagonist, ketanserin diminished the antinociceptive activity of flupirtine, probably due to its additional α1‐adrenoceptor antagonist activity. The antinociceptive effect of clonidine was not influenced by ketanserin. 8 Cholinoceptor antagonists such as mecamylamine and pirenzepine did not alter the antinociceptive action of flupirtine. Flupirtine‐induced antinociception also remained unchanged after pretreatment with haloperidol. 9 Flupirtine has no pharmacologically relevant affinity for α1‐, α2‐adrenoceptors, 5‐HT1‐ and 5‐HT2‐receptors as shown in direct binding studies. 10 The present results indicate that the antinociceptive action induced by flupirtine depends on the descending noradrenergic pain‐modulating system. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Pharmacology Wiley

Mode of antinociceptive action of flupirtine in the rat

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Publisher
Wiley
Copyright
1989 British Pharmacological Society
ISSN
0007-1188
eISSN
1476-5381
D.O.I.
10.1111/j.1476-5381.1989.tb12023.x
Publisher site
See Article on Publisher Site

Abstract

1 Flupirtine is a novel, centrally acting, non‐opioid analgesic agent. The present investigation was undertaken to ascertain which neuronal systems might be responsible for its antinociceptive effect in rodents. The antinociceptive responses to the test compounds were examined in the tail‐flick test. 2 The selective destruction of noradrenergic pathways by 6‐hydroxydopamine considerably reduced the flupirtine‐induced inhibition of nociceptive responses but not the clonidine‐induced antinociception which was significantly enhanced. Depletion of spinal 5‐hydroxytryptaminergic pathways by pretreatment with 5,7‐dihydroxytryptamine failed to affect the action of flupirtine and clonidine. 3 The depletion of neurotransmitters by reserpine totally abolished the antinociceptive action of flupirtine. By contrast, clonidine‐induced inhibition of nociceptive responses remained unchanged. 4 Inhibition of the synthesis of noradrenaline by α‐methyl‐L‐p‐tyrosine attenuated the antinociception induced by flupirtine. In contrast, inhibition of the synthesis of 5‐hydroxytryptamine by (±)−6‐fluorotryptophan did not influence the antinociceptive activity of flupirtine. 5 Inhibition of noradrenaline uptake by imipramine led to a significant augmentation of flupirtine‐induced antinociception. 6 Selective antagonists at α‐adrenoceptors significantly decreased the antinociceptive action of flupirtine. Antinociception induced by clonidine was significantly diminished by idazoxan but not by prazosin. 7 The 5‐hydroxytryptamine (5‐HT) antagonist, ketanserin diminished the antinociceptive activity of flupirtine, probably due to its additional α1‐adrenoceptor antagonist activity. The antinociceptive effect of clonidine was not influenced by ketanserin. 8 Cholinoceptor antagonists such as mecamylamine and pirenzepine did not alter the antinociceptive action of flupirtine. Flupirtine‐induced antinociception also remained unchanged after pretreatment with haloperidol. 9 Flupirtine has no pharmacologically relevant affinity for α1‐, α2‐adrenoceptors, 5‐HT1‐ and 5‐HT2‐receptors as shown in direct binding studies. 10 The present results indicate that the antinociceptive action induced by flupirtine depends on the descending noradrenergic pain‐modulating system.

Journal

British Journal of PharmacologyWiley

Published: Jul 1, 1989

References

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