Mitochondrial DNA multiplex real‐time polymerase chain reaction inhibition assay for quality control of pathogen inactivation by ultraviolet C light in platelet concentrates

Mitochondrial DNA multiplex real‐time polymerase chain reaction inhibition assay for quality... ABBREVIATIONSCtcycle thresholdPC(s)platelet concentrate(s)PIpathogen inactivationPathogen inactivation (PI) technologies for platelet (PLT) products have either been developed or are under active development to reduce the risk of transfusion‐transmitted infection by blood‐borne pathogens. These technologies are also useful for the inactivation of white blood cells (WBCs) that induce transfusion‐associated graft‐versus‐host disease and alloimmunization. Currently available PI systems for PLT products use ultraviolet (UV) light with or without photoactive substances that inactivate pathogens by targeting nucleic acids. The THERAFLEX UV‐Platelets system (Macopharma) is a novel ultraviolet C (UVC)‐based platform that does not include the addition of photoactive substances. Shortwave monochromatic UVC (254 nm) light acts on nucleic acids, thus generating intrastrand or interstrand cyclobutane pyrimidine dimers and/or pyrimidine‐pyrimodone dimers that ultimately prevent replication. Because UVC irradiation mainly affects the nucleic acids of pathogens and WBCs while maintaining plasma and PLT quality, this technology is suitable for the treatment of both plasma and PLT products. UVC treatment significantly reduces the infectivity of plasma and PLT concentrates (PCs) contaminated by disease‐causing viruses, bacteria, and protozoa. The degree of PI correlates with the dose of UVC light delivered.Quality systems are the key to promoting high standards of quality in blood services and to ensuring the availability http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Mitochondrial DNA multiplex real‐time polymerase chain reaction inhibition assay for quality control of pathogen inactivation by ultraviolet C light in platelet concentrates

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Publisher
Wiley
Copyright
© 2018 AABB
ISSN
0041-1132
eISSN
1537-2995
D.O.I.
10.1111/trf.14464
Publisher site
See Article on Publisher Site

Abstract

ABBREVIATIONSCtcycle thresholdPC(s)platelet concentrate(s)PIpathogen inactivationPathogen inactivation (PI) technologies for platelet (PLT) products have either been developed or are under active development to reduce the risk of transfusion‐transmitted infection by blood‐borne pathogens. These technologies are also useful for the inactivation of white blood cells (WBCs) that induce transfusion‐associated graft‐versus‐host disease and alloimmunization. Currently available PI systems for PLT products use ultraviolet (UV) light with or without photoactive substances that inactivate pathogens by targeting nucleic acids. The THERAFLEX UV‐Platelets system (Macopharma) is a novel ultraviolet C (UVC)‐based platform that does not include the addition of photoactive substances. Shortwave monochromatic UVC (254 nm) light acts on nucleic acids, thus generating intrastrand or interstrand cyclobutane pyrimidine dimers and/or pyrimidine‐pyrimodone dimers that ultimately prevent replication. Because UVC irradiation mainly affects the nucleic acids of pathogens and WBCs while maintaining plasma and PLT quality, this technology is suitable for the treatment of both plasma and PLT products. UVC treatment significantly reduces the infectivity of plasma and PLT concentrates (PCs) contaminated by disease‐causing viruses, bacteria, and protozoa. The degree of PI correlates with the dose of UVC light delivered.Quality systems are the key to promoting high standards of quality in blood services and to ensuring the availability

Journal

TransfusionWiley

Published: Jan 1, 2018

References

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