Mitochondrial DNA as a genetic marker for brown trout, Salmo trutta L., populations

Mitochondrial DNA as a genetic marker for brown trout, Salmo trutta L., populations Mitochondrial DNA was examined in natural and hatchery‐reared stocks of brown trout, using different methods of restriction analysis. The methods included the development of a brown trout mt DNA hybridization probe through cloning part of the brown trout mitochondrial genome. In addition, fragments were analysed by ethidium bromide staining and end‐labelling. The relative merits of each of these methods in assessing levels of genetic relatedness between the natural and hatchery‐reared brown trout stocks were evaluated. In addition, the study revealed a diagnostic mtDNA restriction pattern which could be used as a genetic marker for the discrimination of these two groups of brown trout. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Fish Biology Wiley

Mitochondrial DNA as a genetic marker for brown trout, Salmo trutta L., populations

Journal of Fish Biology, Volume 35 (5) – Nov 1, 1989

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Publisher
Wiley
Copyright
Copyright © 1989 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-1112
eISSN
1095-8649
DOI
10.1111/j.1095-8649.1989.tb03020.x
Publisher site
See Article on Publisher Site

Abstract

Mitochondrial DNA was examined in natural and hatchery‐reared stocks of brown trout, using different methods of restriction analysis. The methods included the development of a brown trout mt DNA hybridization probe through cloning part of the brown trout mitochondrial genome. In addition, fragments were analysed by ethidium bromide staining and end‐labelling. The relative merits of each of these methods in assessing levels of genetic relatedness between the natural and hatchery‐reared brown trout stocks were evaluated. In addition, the study revealed a diagnostic mtDNA restriction pattern which could be used as a genetic marker for the discrimination of these two groups of brown trout.

Journal

Journal of Fish BiologyWiley

Published: Nov 1, 1989

References

  • Intra‐ and interspecific variation of the mitochondrial genome in Rattus norvegicus and Rattus rattus : restriction enzyme analysis of variant mitochondrial DNA molecules and their evolutionary relationships
    Brown, Brown; Simpson, Simpson
  • Introgression between two cutthroat trout subspecies with substantial karyotypic, nuclear and mitochondrial genomic divergence
    Gyllensten, Gyllensten; Leary, Leary; Allendorf, Allendorf; Wilson, Wilson

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