MicroRNA395 mediates regulation of sulfate accumulation and allocation in Arabidopsis thaliana

MicroRNA395 mediates regulation of sulfate accumulation and allocation in Arabidopsis thaliana Summary Sulfur is a macronutrient that is necessary for plant growth and development. Sulfate, a major source of sulfur, is taken up by plant roots and transported into various tissues for assimilation. During sulfate limitation, expression of miR395 is significantly up‐regulated. miR395 targets two families of genes, ATP sulfurylases (encoded by APS genes) and sulfate transporter 2;1 (SULTR2;1, also called AST68), both of which are involved in the sulfate metabolism pathway. Their transcripts are suppressed strongly in miR395‐over‐expressing transgenic Arabidopsis, which over‐accumulates sulfate in the shoot but not in the root. APS1 knockdown mutants accumulate twice as much sulfate as the wild‐type. By constructing APS4‐RNAi transgenic plants, we found that silencing the APS4 gene also results in over‐accumulation of sulfate. Even though miR395‐over‐expressing transgenic plants over‐accumulate sulfate in the shoot, they display sulfur deficiency symptoms. Additionally, the distribution of sulfate from older to younger leaves is impaired in miR395‐over‐expressing plants, similar to a SULTR2;1 loss‐of‐function mutant. The aps1‐1 sultr2;1 APS4‐RNAi triply repressed mutants phenocopied miR395‐over‐expressing plants. Our research showed that miR395 is involved in the regulation of sulfate accumulation and allocation by targeting APS genes and SULTR2;1, respectively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

MicroRNA395 mediates regulation of sulfate accumulation and allocation in Arabidopsis thaliana

The Plant Journal, Volume 62 (6) – Jun 1, 2010

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Publisher
Wiley
Copyright
© 2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd
ISSN
0960-7412
eISSN
1365-313X
D.O.I.
10.1111/j.1365-313X.2010.04216.x
Publisher site
See Article on Publisher Site

Abstract

Summary Sulfur is a macronutrient that is necessary for plant growth and development. Sulfate, a major source of sulfur, is taken up by plant roots and transported into various tissues for assimilation. During sulfate limitation, expression of miR395 is significantly up‐regulated. miR395 targets two families of genes, ATP sulfurylases (encoded by APS genes) and sulfate transporter 2;1 (SULTR2;1, also called AST68), both of which are involved in the sulfate metabolism pathway. Their transcripts are suppressed strongly in miR395‐over‐expressing transgenic Arabidopsis, which over‐accumulates sulfate in the shoot but not in the root. APS1 knockdown mutants accumulate twice as much sulfate as the wild‐type. By constructing APS4‐RNAi transgenic plants, we found that silencing the APS4 gene also results in over‐accumulation of sulfate. Even though miR395‐over‐expressing transgenic plants over‐accumulate sulfate in the shoot, they display sulfur deficiency symptoms. Additionally, the distribution of sulfate from older to younger leaves is impaired in miR395‐over‐expressing plants, similar to a SULTR2;1 loss‐of‐function mutant. The aps1‐1 sultr2;1 APS4‐RNAi triply repressed mutants phenocopied miR395‐over‐expressing plants. Our research showed that miR395 is involved in the regulation of sulfate accumulation and allocation by targeting APS genes and SULTR2;1, respectively.

Journal

The Plant JournalWiley

Published: Jun 1, 2010

References

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