MicroRNA‐590‐5p Stabilizes Runx2 by Targeting Smad7 During Osteoblast Differentiation

MicroRNA‐590‐5p Stabilizes Runx2 by Targeting Smad7 During Osteoblast Differentiation ORIGINAL RESEARCH ARTICLE 371 Journal of Journal of Cellular Cellular Physiology Physiology MicroRNA-590-5p Stabilizes Runx2 by Targeting Smad7 During Osteoblast Differentiation 1 1 1 1 1 2 M. VISHAL, S. VIMALRAJ, R. AJEETHA, M. GOKULNATH, R. KEERTHANA, Z. HE, 2 1 N.C. PARTRIDGE, AND N. SELVAMURUGAN * Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India Department of Basic and Craniofacial Biology, New York University College of Dentistry, New York, New York Mesenchymal stem cells (MSCs) are multipotent cells and their differentiation into the osteoblastic lineage is strictly controlled by several regulators, including microRNAs (miRNAs). Runx2 is a bone transcription factor required for osteoblast differentiation. Here, we used in silico analysis to identify a number of miRNAs that putatively target Runx2 and its co-factors to mediate both positive and negative regulation of osteoblast differentiation. Among these miRNAs, miR-590-5p was selected and its expression was found to be increased during osteoblast differentiation. When mouse MSCs (mMSCs) were transiently transfected with a miR-590-5p mimic, we detected an increase in both calcium deposition and the mRNA expression of osteoblast differentiation marker genes such as alkaline phosphatase (ALP) and type I collagen genes. Smad7 was found to be among the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular Physiology Wiley

MicroRNA‐590‐5p Stabilizes Runx2 by Targeting Smad7 During Osteoblast Differentiation

Loading next page...
 
/lp/wiley/microrna-590-5p-stabilizes-runx2-by-targeting-smad7-during-osteoblast-059jOnBXNF
Publisher
Wiley
Copyright
© 2017 Wiley Periodicals, Inc.
ISSN
0021-9541
eISSN
1097-4652
DOI
10.1002/jcp.25434
Publisher site
See Article on Publisher Site

Abstract

ORIGINAL RESEARCH ARTICLE 371 Journal of Journal of Cellular Cellular Physiology Physiology MicroRNA-590-5p Stabilizes Runx2 by Targeting Smad7 During Osteoblast Differentiation 1 1 1 1 1 2 M. VISHAL, S. VIMALRAJ, R. AJEETHA, M. GOKULNATH, R. KEERTHANA, Z. HE, 2 1 N.C. PARTRIDGE, AND N. SELVAMURUGAN * Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India Department of Basic and Craniofacial Biology, New York University College of Dentistry, New York, New York Mesenchymal stem cells (MSCs) are multipotent cells and their differentiation into the osteoblastic lineage is strictly controlled by several regulators, including microRNAs (miRNAs). Runx2 is a bone transcription factor required for osteoblast differentiation. Here, we used in silico analysis to identify a number of miRNAs that putatively target Runx2 and its co-factors to mediate both positive and negative regulation of osteoblast differentiation. Among these miRNAs, miR-590-5p was selected and its expression was found to be increased during osteoblast differentiation. When mouse MSCs (mMSCs) were transiently transfected with a miR-590-5p mimic, we detected an increase in both calcium deposition and the mRNA expression of osteoblast differentiation marker genes such as alkaline phosphatase (ALP) and type I collagen genes. Smad7 was found to be among the

Journal

Journal of Cellular PhysiologyWiley

Published: Feb 1, 2017

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off