ORIGINAL RESEARCH ARTICLE 371 Journal of Journal of Cellular Cellular Physiology Physiology MicroRNA-590-5p Stabilizes Runx2 by Targeting Smad7 During Osteoblast Differentiation 1 1 1 1 1 2 M. VISHAL, S. VIMALRAJ, R. AJEETHA, M. GOKULNATH, R. KEERTHANA, Z. HE, 2 1 N.C. PARTRIDGE, AND N. SELVAMURUGAN * Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India Department of Basic and Craniofacial Biology, New York University College of Dentistry, New York, New York Mesenchymal stem cells (MSCs) are multipotent cells and their differentiation into the osteoblastic lineage is strictly controlled by several regulators, including microRNAs (miRNAs). Runx2 is a bone transcription factor required for osteoblast differentiation. Here, we used in silico analysis to identify a number of miRNAs that putatively target Runx2 and its co-factors to mediate both positive and negative regulation of osteoblast differentiation. Among these miRNAs, miR-590-5p was selected and its expression was found to be increased during osteoblast differentiation. When mouse MSCs (mMSCs) were transiently transfected with a miR-590-5p mimic, we detected an increase in both calcium deposition and the mRNA expression of osteoblast differentiation marker genes such as alkaline phosphatase (ALP) and type I collagen genes. Smad7 was found to be among the
Journal of Cellular Physiology – Wiley
Published: Feb 1, 2017
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