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Mice lacking a functional CHK gene have no apparent defects in the hematopoietic system

Mice lacking a functional CHK gene have no apparent defects in the hematopoietic system Non‐receptor tyrosine kinase Chk has been implicated in hematopoietic development. To study the function of Chk in vivo, we have generated chk‐deficient mice using gene targeting. Overall development of mice homozygous for this mutation was apparently normal. Blood counts, FACS analysis of hematopoietic cell populations, CFU‐C and CAFC assays showed no significant difference between wild type and mutant animals. Thus, the dispensability of Chk for mouse development and hematopoiesis suggests that its function may be redundant in vivo, and most likely be compensated by activity of a closely related protein tyrosine kinase Csk. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png IUBMB Life Wiley

Mice lacking a functional CHK gene have no apparent defects in the hematopoietic system

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 1997 International Union of Biochemistry and Molecular Biology
ISSN
1521-6543
eISSN
1521-6551
DOI
10.1080/15216549700203881
Publisher site
See Article on Publisher Site

Abstract

Non‐receptor tyrosine kinase Chk has been implicated in hematopoietic development. To study the function of Chk in vivo, we have generated chk‐deficient mice using gene targeting. Overall development of mice homozygous for this mutation was apparently normal. Blood counts, FACS analysis of hematopoietic cell populations, CFU‐C and CAFC assays showed no significant difference between wild type and mutant animals. Thus, the dispensability of Chk for mouse development and hematopoiesis suggests that its function may be redundant in vivo, and most likely be compensated by activity of a closely related protein tyrosine kinase Csk.

Journal

IUBMB LifeWiley

Published: Sep 1, 1997

Keywords: Chk; non‐receptor protein tyrosine kinase; gene targeting; flow cytometry; CAFC assay

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