Metagenomics in pooled plasma, with identification of potential emerging infectious pathogens

Metagenomics in pooled plasma, with identification of potential emerging infectious pathogens ABBREVIATIONSEID(s)emerging infectious disease(s)PRpathogen reductionBlood as well as blood products are used as part of supporting medical strategies for saving lives. However, qualified donations are not as safe as previously supposed. Except for routinely tested pathogens such as human immunodeficiency virus, hepatitis B virus, hepatitis C virus, and syphilis, a large number of infectious agents exist in qualified blood, which can impact blood safety and might turn into transfusion‐transmitted diseases. However, there has been no investigation of the diverse pathogens in pooled plasma from manufacturers to date in China for evaluating the blood safety issue caused by undetected pathogens.Hence, we initiated this study to investigate emerging infectious diseases (EIDs) in pooled plasma from manufacturers in Chengdu and Guiyang, China. Random polymerase chain reaction (PCR) and homology testing were used to determine the microbes in the pooled plasma, and the infection status of EIDs that might jeopardize blood safety was then identified.MATERIALS AND METHODSMaterials collection and treatmentEach 12 mL of pooled plasma (8000 donations) from two manufacturers located in Chengdu, Sichuan Province, and Guiyang, Guizhou Province, China, was collected. The samples were first filtered through a 0.45‐μm‐disk filter and second filtered through a 0.22‐μm‐disk filter (Millex GV). The filtered samples were then ultracentrifuged http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Metagenomics in pooled plasma, with identification of potential emerging infectious pathogens

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
© 2018 AABB
ISSN
0041-1132
eISSN
1537-2995
D.O.I.
10.1111/trf.14455
Publisher site
See Article on Publisher Site

Abstract

ABBREVIATIONSEID(s)emerging infectious disease(s)PRpathogen reductionBlood as well as blood products are used as part of supporting medical strategies for saving lives. However, qualified donations are not as safe as previously supposed. Except for routinely tested pathogens such as human immunodeficiency virus, hepatitis B virus, hepatitis C virus, and syphilis, a large number of infectious agents exist in qualified blood, which can impact blood safety and might turn into transfusion‐transmitted diseases. However, there has been no investigation of the diverse pathogens in pooled plasma from manufacturers to date in China for evaluating the blood safety issue caused by undetected pathogens.Hence, we initiated this study to investigate emerging infectious diseases (EIDs) in pooled plasma from manufacturers in Chengdu and Guiyang, China. Random polymerase chain reaction (PCR) and homology testing were used to determine the microbes in the pooled plasma, and the infection status of EIDs that might jeopardize blood safety was then identified.MATERIALS AND METHODSMaterials collection and treatmentEach 12 mL of pooled plasma (8000 donations) from two manufacturers located in Chengdu, Sichuan Province, and Guiyang, Guizhou Province, China, was collected. The samples were first filtered through a 0.45‐μm‐disk filter and second filtered through a 0.22‐μm‐disk filter (Millex GV). The filtered samples were then ultracentrifuged

Journal

TransfusionWiley

Published: Jan 1, 2018

References

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