Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Mechanistic studies on aggregation of polyethylenimine‐DNA complexes and its prevention

Mechanistic studies on aggregation of polyethylenimine‐DNA complexes and its prevention Aggregation of polyethylenimine (PEI)‐DNA complexes severely undermines their utility for gene delivery into mammalian cells. Herein we undertook to elucidate the mechanism of this deleterious phenomenon and to develop rational strategies for its prevention. The effect of temperature, surfactants, complex concentration, ionic strength, viscosity, and pH on the time course of this aggregation was systematically examined. The aggregation process was completely inhibited by 2.5% polyoxyethylene (100) stearate (POES) and to a lesser degree by other nonionic surfactants. Importantly, POES preserved the transfection efficiency of the complexes without inducing toxicity. The aggregation was also reduced by lowering the temperature and pH, diluting the complexes, and increasing the solution viscosity. It is concluded that PEI‐DNA complexes aggregate primarily due to hydrophobic interactions, while electrostatic attractions play little role. © 2005 Wiley Periodicals, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

Mechanistic studies on aggregation of polyethylenimine‐DNA complexes and its prevention

Loading next page...
 
/lp/wiley/mechanistic-studies-on-aggregation-of-polyethylenimine-dna-complexes-l630FmDVRL

References (31)

Publisher
Wiley
Copyright
Copyright © 2005 Wiley Periodicals, Inc., A Wiley Company
ISSN
0006-3592
eISSN
1097-0290
DOI
10.1002/bit.20444
pmid
15818564
Publisher site
See Article on Publisher Site

Abstract

Aggregation of polyethylenimine (PEI)‐DNA complexes severely undermines their utility for gene delivery into mammalian cells. Herein we undertook to elucidate the mechanism of this deleterious phenomenon and to develop rational strategies for its prevention. The effect of temperature, surfactants, complex concentration, ionic strength, viscosity, and pH on the time course of this aggregation was systematically examined. The aggregation process was completely inhibited by 2.5% polyoxyethylene (100) stearate (POES) and to a lesser degree by other nonionic surfactants. Importantly, POES preserved the transfection efficiency of the complexes without inducing toxicity. The aggregation was also reduced by lowering the temperature and pH, diluting the complexes, and increasing the solution viscosity. It is concluded that PEI‐DNA complexes aggregate primarily due to hydrophobic interactions, while electrostatic attractions play little role. © 2005 Wiley Periodicals, Inc.

Journal

Biotechnology and BioengineeringWiley

Published: Jun 5, 2005

Keywords: nonviral gene delivery; plasmid DNA; polyethylenimine; aggregation; surfactants; stabilization

There are no references for this article.