MEASUREMENTS OF IN VIVO RATES OF PROTEIN SYNTHESIS IN BRAIN, SPINAL CORD, HEART AND LIVER OF YOUNG VERSUS ADULT RATS, INTACT VERSUS HYPOPHYSECTOMIZED RATS

MEASUREMENTS OF IN VIVO RATES OF PROTEIN SYNTHESIS IN BRAIN, SPINAL CORD, HEART AND LIVER OF... Abstract— In vivo protein synthesis rates in rats were estimated by single i.p. injections of large quantities of (1‐14C)valine. This method gives reliable estimates of the precursor specific activity and average protein synthesis rates. In the brain, spinal cord, heart and liver, the average rates for adults were 0.65, 0.42, 0.49 and 1.92% replacement of protein‐bound amino acid per h. In the brain and liver of 10‐day olds the average rates were estimated to be 1.46 and 3.12% per h respectively. Hypophysectomy decreased synthesis rates by 25% or more in all tissues studied. The disadvantages of the method are that applying large amounts of valine i.p. appeared to constitute a stress and that the valine solution required for injection was hypertonic, causing withdrawal of body fluids of the animal. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

MEASUREMENTS OF IN VIVO RATES OF PROTEIN SYNTHESIS IN BRAIN, SPINAL CORD, HEART AND LIVER OF YOUNG VERSUS ADULT RATS, INTACT VERSUS HYPOPHYSECTOMIZED RATS

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Publisher
Wiley
Copyright
Copyright © 1978 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1978.tb07812.x
Publisher site
See Article on Publisher Site

Abstract

Abstract— In vivo protein synthesis rates in rats were estimated by single i.p. injections of large quantities of (1‐14C)valine. This method gives reliable estimates of the precursor specific activity and average protein synthesis rates. In the brain, spinal cord, heart and liver, the average rates for adults were 0.65, 0.42, 0.49 and 1.92% replacement of protein‐bound amino acid per h. In the brain and liver of 10‐day olds the average rates were estimated to be 1.46 and 3.12% per h respectively. Hypophysectomy decreased synthesis rates by 25% or more in all tissues studied. The disadvantages of the method are that applying large amounts of valine i.p. appeared to constitute a stress and that the valine solution required for injection was hypertonic, causing withdrawal of body fluids of the animal.

Journal

Journal of NeurochemistryWiley

Published: Mar 1, 1978

References

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