Measurement of excreted steroids in Macaca nemestrina

Measurement of excreted steroids in Macaca nemestrina A practical method for the quantitative measurement of the estrogenic steroid estradiol‐17β in the feces of pigtailed macaques (Macaca nemestrina) is described. The method, which includes homogenization, filtration, ether extraction, and sephadex purification, produces an 85.3% recovery of 3H‐estradiol. Comparable recovery of 3H‐progesterone (64.1%) and 3H‐testoster‐one (67.9%) is also obtained. Estradiol, measured by radioimmunoassay methodology, has inter‐ and intra‐assay variations of 7.1% and 4.3%, respectively. Linearity of estradiol was checked by varying the amount of feces analyzed. Results show a correlation of 0.9945, Y = 32.526x + 0.205. Accuracy of estradiol, determined by adding radioinert estradiol to the feces before homogenization, was 98.7% with a correlation of 0.9950, Y = 1.027x − 0.543. The menstrual cycles of several animals were analyzed and corresponded well with serum levels of estradiol‐17β. Similar correspondence was obtained in matched fecal and serum samples from pregnant animals. These methods provide a practical and accurate solution to the problem of collecting hormone data in field studies without the potential complications of capture in laboratory research. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Primatology Wiley

Measurement of excreted steroids in Macaca nemestrina

Loading next page...
 
/lp/wiley/measurement-of-excreted-steroids-in-macaca-nemestrina-pnnZD8zGFn
Publisher
Wiley
Copyright
Copyright © 1987 Wiley‐Liss, Inc., A Wiley Company
ISSN
0275-2565
eISSN
1098-2345
D.O.I.
10.1002/ajp.1350120105
Publisher site
See Article on Publisher Site

Abstract

A practical method for the quantitative measurement of the estrogenic steroid estradiol‐17β in the feces of pigtailed macaques (Macaca nemestrina) is described. The method, which includes homogenization, filtration, ether extraction, and sephadex purification, produces an 85.3% recovery of 3H‐estradiol. Comparable recovery of 3H‐progesterone (64.1%) and 3H‐testoster‐one (67.9%) is also obtained. Estradiol, measured by radioimmunoassay methodology, has inter‐ and intra‐assay variations of 7.1% and 4.3%, respectively. Linearity of estradiol was checked by varying the amount of feces analyzed. Results show a correlation of 0.9945, Y = 32.526x + 0.205. Accuracy of estradiol, determined by adding radioinert estradiol to the feces before homogenization, was 98.7% with a correlation of 0.9950, Y = 1.027x − 0.543. The menstrual cycles of several animals were analyzed and corresponded well with serum levels of estradiol‐17β. Similar correspondence was obtained in matched fecal and serum samples from pregnant animals. These methods provide a practical and accurate solution to the problem of collecting hormone data in field studies without the potential complications of capture in laboratory research.

Journal

American Journal of PrimatologyWiley

Published: Jan 1, 1987

References

  • Nonchromatographic radioimmunoassay of unconjugated estriol in plasma, with polyethylene glycol as precipitant
    Schiller, Schiller; Brammall, Brammall

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off