MALDI‐MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation

MALDI‐MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella... In many amphibians, the granular glands can be grouped in special regions forming macroglands. This is the case of toads, characterized by the presence of a pair of parotoid macroglands, strategically located to give protection by poison release in case of attacks. The product secreted consists of a wide variety of chemical compounds including proteins, peptides, biogenic amines, toxic steroidal bufadienolides, and various alkaloids, depending on the species. In this work, using Rhinella arenarum, we have performed, for the first time, the matrix assisted‐ultraviolet laser desorption/ionization mass spectrometry and tandem mass spectrometry characterization of the components of the secretion used as crude material, just suspended in MeOH (or MeCN). The crude sample as a whole (whole suspension) was spotted on the matrix assisted‐ultraviolet laser desorption plate for analysis. Electrospray ionization‐Orbitrap was used for cross‐checking experiments. The pattern of signals obtained at m/z ranges 600 to 800 and 1200 to 1600 could be assigned as the argininyl bufadienolide esters fingerprint characteristic of female and male. Variation patterns for gender (female, male), age (non‐reproductive, reproductive), and season (non‐reproductive, reproductive) are described. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Mass Spectrometry (Incorp Biological Mass Spectrometry) Wiley

MALDI‐MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons, Ltd.
ISSN
1076-5174
eISSN
1096-9888
D.O.I.
10.1002/jms.4082
Publisher site
See Article on Publisher Site

Abstract

In many amphibians, the granular glands can be grouped in special regions forming macroglands. This is the case of toads, characterized by the presence of a pair of parotoid macroglands, strategically located to give protection by poison release in case of attacks. The product secreted consists of a wide variety of chemical compounds including proteins, peptides, biogenic amines, toxic steroidal bufadienolides, and various alkaloids, depending on the species. In this work, using Rhinella arenarum, we have performed, for the first time, the matrix assisted‐ultraviolet laser desorption/ionization mass spectrometry and tandem mass spectrometry characterization of the components of the secretion used as crude material, just suspended in MeOH (or MeCN). The crude sample as a whole (whole suspension) was spotted on the matrix assisted‐ultraviolet laser desorption plate for analysis. Electrospray ionization‐Orbitrap was used for cross‐checking experiments. The pattern of signals obtained at m/z ranges 600 to 800 and 1200 to 1600 could be assigned as the argininyl bufadienolide esters fingerprint characteristic of female and male. Variation patterns for gender (female, male), age (non‐reproductive, reproductive), and season (non‐reproductive, reproductive) are described.

Journal

Journal of Mass Spectrometry (Incorp Biological Mass Spectrometry)Wiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

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