Light sheet microscopy and SrAl2O4 nanoparticles codoped with Eu2+/Dy3+ ions for cancer cell tagging

Light sheet microscopy and SrAl2O4 nanoparticles codoped with Eu2+/Dy3+ ions for cancer cell tagging Light sheet optical microscopy on strontium aluminate nanoparticles (SrAl2O4 NPs)1 codoped with Eu2+ and Dy3+ was used for cancer cell tagging and tracking. The nanoparticles were synthesized by urea‐assisted combustion with optimized percentage values of the 2 codoping rare‐earth ions for cell viability and for lower cytotoxic effects. The optical properties of these materials showed an excitation wide range of wavelengths (λexc = 254‐460 nm), a broad emission band (λem = 475‐575 nm) with the maximum centered wavelength at 525 nm and a half lifetime within the seconds regime. The feasibility to measure the nanoparticle luminescence under the selective plane illumination configuration was studied by immersing the nanoparticles in 1% Agarose. The potential applicability of the synthesized nanophosphors for cancer cell tagging was demonstrated by using in vitro experiments with human breast adenocarcinoma MCF‐7 cells. A single MCF‐7 cell observed by the use of light sheet microscopy with UV excitation. The cell has been bio‐labeled with FA‐SrAl204: Eu2+, Dy3+ NPs and 4′,6‐diamidino‐2‐phenylindole, dihydrochloride for nucleus identification. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biophotonics Wiley

Light sheet microscopy and SrAl2O4 nanoparticles codoped with Eu2+/Dy3+ ions for cancer cell tagging

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
1864-063X
eISSN
1864-0648
D.O.I.
10.1002/jbio.201700301
Publisher site
See Article on Publisher Site

Abstract

Light sheet optical microscopy on strontium aluminate nanoparticles (SrAl2O4 NPs)1 codoped with Eu2+ and Dy3+ was used for cancer cell tagging and tracking. The nanoparticles were synthesized by urea‐assisted combustion with optimized percentage values of the 2 codoping rare‐earth ions for cell viability and for lower cytotoxic effects. The optical properties of these materials showed an excitation wide range of wavelengths (λexc = 254‐460 nm), a broad emission band (λem = 475‐575 nm) with the maximum centered wavelength at 525 nm and a half lifetime within the seconds regime. The feasibility to measure the nanoparticle luminescence under the selective plane illumination configuration was studied by immersing the nanoparticles in 1% Agarose. The potential applicability of the synthesized nanophosphors for cancer cell tagging was demonstrated by using in vitro experiments with human breast adenocarcinoma MCF‐7 cells. A single MCF‐7 cell observed by the use of light sheet microscopy with UV excitation. The cell has been bio‐labeled with FA‐SrAl204: Eu2+, Dy3+ NPs and 4′,6‐diamidino‐2‐phenylindole, dihydrochloride for nucleus identification.

Journal

Journal of BiophotonicsWiley

Published: Jan 1, 2018

Keywords: ; ; ;

References

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