Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Laser Raman studies of conformational variations of poly‐ L ‐lysine

Laser Raman studies of conformational variations of poly‐ L ‐lysine The frequencies and intensities of the laser Raman spectra of poly‐L‐lysine (PLL) have been observed in the following studies: (1) the thermally induced α‐to‐β transition which occurs with increasing temperature at high pH; (2) the ionized form to α transition at 10°C by increasing pH; and (3) the ionized form to α transition by ionic strength at low pH. The frequency‐dependent bands which have been observed are the amide I (in H2O), amide I′ (in D2O), amide III, and C–C stretch. It has been found possible to assign an unique set of frequencies and intensities to each conformation of PLL of α, β, and ionized form. In this way the nature of the conformations intermediate in the transitions can be determined. The frequencies of the amide III and amide III′ are very weak in the α‐helix and somewhat higher than usual in the β form. Hence it appears the amide III and amide III′ bands may differ from one type of polypeptide to another with the same backbone conformation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biopolymers Wiley

Laser Raman studies of conformational variations of poly‐ L ‐lysine

Loading next page...
 
/lp/wiley/laser-raman-studies-of-conformational-variations-of-poly-l-lysine-UfteazDyEn

References (23)

Publisher
Wiley
Copyright
Copyright © 1973 John Wiley & Sons, Inc.
ISSN
0006-3525
eISSN
1097-0282
DOI
10.1002/bip.1973.360120919
pmid
4744756
Publisher site
See Article on Publisher Site

Abstract

The frequencies and intensities of the laser Raman spectra of poly‐L‐lysine (PLL) have been observed in the following studies: (1) the thermally induced α‐to‐β transition which occurs with increasing temperature at high pH; (2) the ionized form to α transition at 10°C by increasing pH; and (3) the ionized form to α transition by ionic strength at low pH. The frequency‐dependent bands which have been observed are the amide I (in H2O), amide I′ (in D2O), amide III, and C–C stretch. It has been found possible to assign an unique set of frequencies and intensities to each conformation of PLL of α, β, and ionized form. In this way the nature of the conformations intermediate in the transitions can be determined. The frequencies of the amide III and amide III′ are very weak in the α‐helix and somewhat higher than usual in the β form. Hence it appears the amide III and amide III′ bands may differ from one type of polypeptide to another with the same backbone conformation.

Journal

BiopolymersWiley

Published: Sep 1, 1973

There are no references for this article.