laboratory diagnosis of parvovirus B19 infection

laboratory diagnosis of parvovirus B19 infection The sensitivity and application of the polymerase chain reaction (PCR) for the diagnosis of parvovirus B19 (B19) infection was investigated by simultaneously assaying a collection of 279 consecutively received samples for presence of anti‐B19 IgM and IgG antibodies by Western blot and for B19 DNA by PCR and dot‐blot hybridization (dot‐blot); samples were sera from patients with suspected B19 infection. PCR and dot‐blot detected B19 DNA in 9% (16/179) and 1% (2/179), respectively of Ab‐positive samples (IgM+/IgG‐,IgM+IgG+,IgM‐IgG+), and in 28% (15/54) and 2% (1/54), respectively, of IgM+ samples. PCR also detected B19 DNA in 2% (2/100) of IgM‐/IgG‐ samples, both of which had normal total IgG and IgM levels. PCR is of unique value because it permits diagnosis of B19 infection even in the absence of specific acute phase (IgM) and in the presence or absence of convalescentphase (IgG) Ab. © 1992 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Laboratory Analysis Wiley

laboratory diagnosis of parvovirus B19 infection

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Publisher
Wiley
Copyright
Copyright © 1992 Wiley Periodicals, Inc., A Wiley Company
ISSN
0887-8013
eISSN
1098-2825
DOI
10.1002/jcla.1860060402
Publisher site
See Article on Publisher Site

Abstract

The sensitivity and application of the polymerase chain reaction (PCR) for the diagnosis of parvovirus B19 (B19) infection was investigated by simultaneously assaying a collection of 279 consecutively received samples for presence of anti‐B19 IgM and IgG antibodies by Western blot and for B19 DNA by PCR and dot‐blot hybridization (dot‐blot); samples were sera from patients with suspected B19 infection. PCR and dot‐blot detected B19 DNA in 9% (16/179) and 1% (2/179), respectively of Ab‐positive samples (IgM+/IgG‐,IgM+IgG+,IgM‐IgG+), and in 28% (15/54) and 2% (1/54), respectively, of IgM+ samples. PCR also detected B19 DNA in 2% (2/100) of IgM‐/IgG‐ samples, both of which had normal total IgG and IgM levels. PCR is of unique value because it permits diagnosis of B19 infection even in the absence of specific acute phase (IgM) and in the presence or absence of convalescentphase (IgG) Ab. © 1992 Wiley‐Liss, Inc.

Journal

Journal of Clinical Laboratory AnalysisWiley

Published: Jan 1, 1992

References

  • Anemia caused by parvovirus in an adult patient with acute lymphoblastic leukemia in complete remission
    Takahashi, Takahashi; Moriyama, Moriyama; Shibata, Shibata; Takai, Takai
  • Detection of human parvovirus using a molecularly cloned probe
    Clewley, Clewley
  • Diagnosis of human parvovirus infection by dot‐blot hybridization using cloned viral DNA
    Anderson, Anderson; Jones, Jones; Minson, Minson

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