KINETICS OF BLOOD‐BRAIN BARRIER TRANSPORT OF PYRUVATE, LACTATE AND GLUCOSE IN SUCKLING, WEANLING AND ADULT RATS

KINETICS OF BLOOD‐BRAIN BARRIER TRANSPORT OF PYRUVATE, LACTATE AND GLUCOSE IN SUCKLING,... Abstract— The kinetics of the uptake from blood to brain of pyruvate, lactate and glucose have been determined in rats of different ages. The carotid artery single injection technique was used in animals anaesthetized with pentobarbital. The rates of influx for each substrate were determined over a range of concentrations for the different age‐groups. Data were analysed in terms of the Michaelis‐Menten equation with a component to allow for non‐saturable diffusion. Values are given for Km, Vmax and Kd. In suckling rats (15‐21 days) the Vmax values for both pyruvate and lactate were 2.0 μmol g−1 min−1. In 28‐day‐old rats the Vmax values had fallen to one‐half and in adults they were less than one‐tenth. Km, values were higher in the younger animals. The rate of glucose transport in suckling rats was half that of 28‐day‐old and adults although there was no difference with age in the Km values. The results are discussed in relation to the net flux of these substrates in and out of brain during different stages of post‐natal development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

KINETICS OF BLOOD‐BRAIN BARRIER TRANSPORT OF PYRUVATE, LACTATE AND GLUCOSE IN SUCKLING, WEANLING AND ADULT RATS

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Publisher
Wiley
Copyright
Copyright © 1979 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1979.tb05173.x
Publisher site
See Article on Publisher Site

Abstract

Abstract— The kinetics of the uptake from blood to brain of pyruvate, lactate and glucose have been determined in rats of different ages. The carotid artery single injection technique was used in animals anaesthetized with pentobarbital. The rates of influx for each substrate were determined over a range of concentrations for the different age‐groups. Data were analysed in terms of the Michaelis‐Menten equation with a component to allow for non‐saturable diffusion. Values are given for Km, Vmax and Kd. In suckling rats (15‐21 days) the Vmax values for both pyruvate and lactate were 2.0 μmol g−1 min−1. In 28‐day‐old rats the Vmax values had fallen to one‐half and in adults they were less than one‐tenth. Km, values were higher in the younger animals. The rate of glucose transport in suckling rats was half that of 28‐day‐old and adults although there was no difference with age in the Km values. The results are discussed in relation to the net flux of these substrates in and out of brain during different stages of post‐natal development.

Journal

Journal of NeurochemistryWiley

Published: Aug 1, 1979

References

  • Estimation of kinetic parameters of unidirectional transport across the blood‐brain barrier
    Cunningham, Cunningham; Sarna, Sarna
  • The effect of age upon the influx of glucose into the brain
    Daniel, Daniel; Love, Love; Pratt, Pratt
  • Neutral amino acids in the brain: changes in response to food ingestion
    Fernstrom, Fernstrom; Faller, Faller
  • Transport of metabolic substrates through the blood‐brain barrier
    Pardridge, Pardridge; Oldendorf, Oldendorf

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