Guidelines for the use of flow cytometry and cell sorting in immunological studiesThe October 2017 issue of the European Journal of Immunology (EJI) featured the ‘Guidelines for the use of flow cytometry and cell sorting in immunological studies’, an over 200 pages long article that not only highlights the latest trends and best practices in basic and advanced flow cytometry, but also re‐enforces optimal sample preparation and data analysis. The “Guidelines project” started from the observation that flow cytometry is a fundamental and continuously evolving technique in immunology, however, many flow cytometry experiments are not correctly performed. Written by immunologists to immunologists, the “Guidelines” are introduced as a comprehensive flow cytometry resource covering a wide range of applications, form cell sorting to multiparameter and rare‐cell analysis, including bar coding, CyTOF and imaging cytometry. The final aim of the “Guidelines” is to help the community to ensure that the gating strategies employed are fully valid and to enable the correct use of state of the art flow cytometry.Cossarizza et al. Eur J Immunol 2017; 47. DOI: 10.1002/eji.201646632Types of cell death and apoptotic stages in Chinese Hamster Ovary cells distinguished by Raman spectroscopyCell death is the ultimate cause of productivity loss in bioreactors that are used to produce therapeutic proteins. In this Biotechnology and Bioengineering paper by Rangan and coworkers published in the February 2018 Issue, Raman spectroscopy successfully distinguished apoptotic and necrotic cell death in Chinese hamster ovary cells. the stages of apoptosis were also successfully identified; in particular, it was shown that the onset of apoptosis could be detected earlier than conventional annexin V staining. These results indicate that Raman spectroscopy is a promising tool for detecting cell state and the onset of cell death in bioreactor.Rangan et al. Biotechnol. Bioeng. 2018; 115(2). DOI: 10.1002/bit.26476
Cytometry – Wiley
Published: Jan 1, 2018
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