Isolation of pike fry rhabdovirus from Pseudorasbora parva (Temminck & Schlegel)

Isolation of pike fry rhabdovirus from Pseudorasbora parva (Temminck & Schlegel) W. AHNE & I. THOMSEN Institute of Zoology and Hydrobiology, University of Munich, Federal Republic of Germany Thepikefry rhabdovirus (PFR) which causes an acute disease of pike fry, Esox lucius L., (de Kinkelin, Galimard & Bootsma 1973) has also been isolated from: grass carp, Ctenopharyngodon idella (Valenciennes) (Ahne 1975); tench. Tinea tinea L.; and white bream, Blicca bjoerkna (L.) (Ahne, Mahnel & Steinhagen 1982). This paper describes the isolation of PFR from Pseudorasbora parva (Temminck & Schlegel), an ornamental fish native to East Asia. This fish was introduced in Europe by accident together with the Chinese grass carp in 1960. The gudgeon is now widespread in the wild in Europe and it is, to some extent, popular in fish farms. Pseudorasbora of about 1-5 g were examined for diseases using routine diagnostic procedures. The fish examined did not show any signs of disease. For virological investigations, pools of kidney, liver and spleen tissue were homogenized, diluted 1:10 in PBS, centrifuged (3000 g for 20 min) and the filtered (300 nm membrane filter) supernatant was inoculated on to fathead minnow (FHM) cells. Infected cells incubated at 20°C showed focal areas of cytopathic effect (lysis) 3 days after infection. The http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Fish Diseases Wiley

Isolation of pike fry rhabdovirus from Pseudorasbora parva (Temminck & Schlegel)

Journal of Fish Diseases, Volume 9 (6) – Nov 1, 1986

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Publisher
Wiley
Copyright
Copyright © 1986 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0140-7775
eISSN
1365-2761
D.O.I.
10.1111/j.1365-2761.1986.tb01053.x
Publisher site
See Article on Publisher Site

Abstract

W. AHNE & I. THOMSEN Institute of Zoology and Hydrobiology, University of Munich, Federal Republic of Germany Thepikefry rhabdovirus (PFR) which causes an acute disease of pike fry, Esox lucius L., (de Kinkelin, Galimard & Bootsma 1973) has also been isolated from: grass carp, Ctenopharyngodon idella (Valenciennes) (Ahne 1975); tench. Tinea tinea L.; and white bream, Blicca bjoerkna (L.) (Ahne, Mahnel & Steinhagen 1982). This paper describes the isolation of PFR from Pseudorasbora parva (Temminck & Schlegel), an ornamental fish native to East Asia. This fish was introduced in Europe by accident together with the Chinese grass carp in 1960. The gudgeon is now widespread in the wild in Europe and it is, to some extent, popular in fish farms. Pseudorasbora of about 1-5 g were examined for diseases using routine diagnostic procedures. The fish examined did not show any signs of disease. For virological investigations, pools of kidney, liver and spleen tissue were homogenized, diluted 1:10 in PBS, centrifuged (3000 g for 20 min) and the filtered (300 nm membrane filter) supernatant was inoculated on to fathead minnow (FHM) cells. Infected cells incubated at 20°C showed focal areas of cytopathic effect (lysis) 3 days after infection. The

Journal

Journal of Fish DiseasesWiley

Published: Nov 1, 1986

References

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