Isolation of allicepin, a novel antifungal peptide from onion (Allium cepa) bulbs

Isolation of allicepin, a novel antifungal peptide from onion (Allium cepa) bulbs From the bulbs of the onion Allium cepa, a novel antifungal peptide distinct from the antimicrobial peptide previously reported from onion seeds was isolated. The antifungal peptide, designated allicepin, was purified with a procedure that involved aqueous extraction, ion exchange chromatography on DEAE‐cellulose, affinity chromatography on Affi‐gel blue gel and FPLC‐gel filtration on Superdex 75. Allicepin was unadsorbed on DEAE‐cellulose and adsorbed on Affi‐gel blue gel. The molecular weight of allicepin was estimated to be 10 K by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and gel filtration on Superdex 75. Allicepin exerted an inhibitory activity on mycelial growth in several fungal species including Botrytis cinerea, Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Peptide Science Wiley

Isolation of allicepin, a novel antifungal peptide from onion (Allium cepa) bulbs

Journal of Peptide Science, Volume 10 (3) – Jan 1, 2004

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Publisher
Wiley
Copyright
Copyright © 2004 Wiley Subscription Services
ISSN
1075-2617
eISSN
1099-1387
D.O.I.
10.1002/psc.509
Publisher site
See Article on Publisher Site

Abstract

From the bulbs of the onion Allium cepa, a novel antifungal peptide distinct from the antimicrobial peptide previously reported from onion seeds was isolated. The antifungal peptide, designated allicepin, was purified with a procedure that involved aqueous extraction, ion exchange chromatography on DEAE‐cellulose, affinity chromatography on Affi‐gel blue gel and FPLC‐gel filtration on Superdex 75. Allicepin was unadsorbed on DEAE‐cellulose and adsorbed on Affi‐gel blue gel. The molecular weight of allicepin was estimated to be 10 K by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and gel filtration on Superdex 75. Allicepin exerted an inhibitory activity on mycelial growth in several fungal species including Botrytis cinerea, Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd.

Journal

Journal of Peptide ScienceWiley

Published: Jan 1, 2004

Keywords: ; ; ;

References

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