Quantitative and qualitative determinations of phenolic compounds were carried out on Chemlali olive leaves, a by‐product of olive tree pruning. The evolution of the amount of the major phenolic compound was monitored from July 2003 to March 2004. Quantitative analysis was performed by high‐performance liquid chromatography (HPLC), which revealed that oleuropein was always the major compound. Its concentration reached 6.8 g per 100 g of fresh olive leaves, while monomeric phenols were the minor compounds. Beside oleuropein, six flavonoids (luteolin 7‐O‐glucoside, luteolin 7‐O‐rutinoside, apigenin 7‐O‐glucoside, rutin, luteolin and apigenin) were identified. The identification was based on separation by HPLC equipped with a diode array detector, followed by liquid chromatography‐mass spectrometry analysis. A relative high amount of purified hydroxytyrosol (2.3 g per 100 g of fresh olive leaves) was obtained in short time by a simple hydrolysis reaction of Olea europaea leaf extract and by purification using a C‐18 silica gel column. The antioxidant activities of the extracts and the purified compounds were evaluated by measuring the radical‐scavenging effect on 1,1‐diphenyl‐2‐picrylhydrazyl and by using the β‐carotene linoleate model assay. Hydroxytyrosol and hydrolysate products have the highest antioxidant activities.
European Journal of Lipid Science and Technology – Wiley
Published: Aug 1, 2005
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera